Table 1. Model parameters and properties... | Rockefeller University Press

Table 1.

Model parameters and properties

	Parameters									Properties
	$k_{2, 1}^{0}$ (s⁻¹)	$k_{2, 1}^{1}$ (mV⁻¹)	$k_{2, 3}^{0}$ (s⁻¹)	$k_{2, 3}^{1}$ (mV⁻¹)	$k_{3, 4}^{0}$ (s⁻¹)	$k_{4, 3}^{0}$ (s⁻¹)	$k_{4, 3}^{1}$ (mV⁻¹)	a₁	N_C	P_O	f_R
True	100.00	−0.13	5,000.00	0.02	3,000.00	5.00	−0.01	2.0	5,000	0.4175	0.4292
Initial	100.00	−0.075	1,500.00	0.05	1,500.00	20.00	−0.10	3.0	3,000	0.3198	1.0
Run I	77.08	−0.14	4,841.62	0.02	3,102.51	11.75	0.01	1.99	5,073	0.4062	0.1843
Run II	91.18	−0.13	4,460.68	0.02	3,458.43	9.64	0.00	2.22	5,667	0.3723	0.3946
Run III	82.75	−0.13	3,976.55	0.02	4,129.05	7.24	0.00	2.05	6,623^{^a}	0.3125	0.3543
Run IV	95.39	−0.13	6,056.44	0.02	2,414.50	7.52	0.00	1.90	4,061	0.4992^{^a}	0.3186
Run V	98.88	−0.13	5,039.69	0.02	3,001.74	2.52	−0.03	1.81	4,919	0.4135	0.7991^{^a}
Run VI	112.61	−0.13	6,280.71	0.02	2,416.81	1.88	−0.04	1.72	4,055	0.4995^{^a}	0.7998^{^a}

	Parameters									Properties
	$k_{2, 1}^{0}$ (s⁻¹)	$k_{2, 1}^{1}$ (mV⁻¹)	$k_{2, 3}^{0}$ (s⁻¹)	$k_{2, 3}^{1}$ (mV⁻¹)	$k_{3, 4}^{0}$ (s⁻¹)	$k_{4, 3}^{0}$ (s⁻¹)	$k_{4, 3}^{1}$ (mV⁻¹)	a₁	N_C	P_O	f_R
True	100.00	−0.13	5,000.00	0.02	3,000.00	5.00	−0.01	2.0	5,000	0.4175	0.4292
Initial	100.00	−0.075	1,500.00	0.05	1,500.00	20.00	−0.10	3.0	3,000	0.3198	1.0
Run I	77.08	−0.14	4,841.62	0.02	3,102.51	11.75	0.01	1.99	5,073	0.4062	0.1843
Run II	91.18	−0.13	4,460.68	0.02	3,458.43	9.64	0.00	2.22	5,667	0.3723	0.3946
Run III	82.75	−0.13	3,976.55	0.02	4,129.05	7.24	0.00	2.05	6,623^{^a}	0.3125	0.3543
Run IV	95.39	−0.13	6,056.44	0.02	2,414.50	7.52	0.00	1.90	4,061	0.4992^{^a}	0.3186
Run V	98.88	−0.13	5,039.69	0.02	3,001.74	2.52	−0.03	1.81	4,919	0.4135	0.7991^{^a}
Run VI	112.61	−0.13	6,280.71	0.02	2,416.81	1.88	−0.04	1.72	4,055	0.4995^{^a}	0.7998^{^a}

The quantities refer to the kinetic model shown in Fig. 2 A. $k_{ij}^{0}$ and $k_{ij}^{1}$ represent rate constant parameters, as defined by Eq. 1 from the companion paper (Salari et al., 2018; rate constant $k_{ij} = k_{ij}^{0} \times e^{k_{ij}^{1} \times V}$ ⁠), a₁ is an allosteric factor, and N_C is the channel count. P_o and f_R represent model properties, as defined in Fig. 2 B. The “true” parameter values were used to simulate the data shown in Fig. 3. The “initial” and the “run” values refer to the starting and the ending of optimization, respectively, as plotted in Figs. 4 and 5. Some rate constant parameters are not shown, because they are defined by constraints (e.g., $k_{1, 2}^{0} = a_{1} \times k_{2, 3}^{0}$ ⁠) and can be easily derived.

Optimization runs where model parameters and properties were constrained away from the true values.

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