Table 1.
Sequence of primers used for cloning TCav3 cDNA and semiquantitative RT-PCR
PrimerSequence (5′ to 3′)
TCav3 NT cDNA CTTTAGGTAGTATGAGCAAGGAATG 
TCav3 CT cDNA TTTTTTTTTTTTTTTTTTVN 
TCav3 NT 5′1 TGATGTTTTATTCAAGTCATGC 
TCav3 NT 5′2 TACTTAGCTAGCCGCGGGAGCCACCATGGATATTTCGTTCGCTATCAT 
TCav3 NT 3′1 CATCAGGCGTCTTAACTTTGC 
TCav3 NT 3′2 TGCTCTGTGGTGGATCTCGAG 
TCav3 CT 3′1 CTTGATATTGATTAAATTTATCCAGATG 
TCav3 CT 3′2 ACTCATCCCGGGTTATACTAATGTTTGAATCAT 
TCav3 CT 5′1 AAATCTGGTAGATCCTAATGAAGTC 
TCav3 CT 5′2 TCGCGTTCCAATTGGTCCTCGAG 
TCav1_F AGTCTTTACCGATTTGGCTCTTCTTTG 
TCav1_R CAATTATACGACTGACACTCTTTCAAAGC 
TCav2_F CGTTTACAAAAGTCTGGATCAAGTTGCG 
TCav2_R CTGCTGTACATTTTGATATGTTAGTAAGATCATCTG 
TCav3_F GTAGAAGATCAGATGGAATTAACGGCTTCC 
TCav3_R TACTAATGTTTGAATCATTTTTCGAGGTAATGTGACC 
TCavβ_F AGTAATAAATTCTCAGGATTAGTGGAAGTGTCC 
TCavβ_R AACATCATTACGTTTTATTACTAGAGGATCTTG 
TCavα2δ-a_F CTCAAGCAGTGGACTAACTAAACTTTACC 
TCavα2δ-a_R CATAGCTGGTTATATGTCACAAATTCCTCTC 
TCavα2δ-b_F TTTACCACATCCTTCTTCAGCTTCTTGAC 
TCavα2δ-b_R AGCATACACTGATAGCTTTACATAGCG 
TCavα2δ-c_F GATATTCGAAATTTCGAATGATACCCTTTGC 
TCavα2δ-c-R CAACGCGAGAAGTAGAAGTGTCCG 
PrimerSequence (5′ to 3′)
TCav3 NT cDNA CTTTAGGTAGTATGAGCAAGGAATG 
TCav3 CT cDNA TTTTTTTTTTTTTTTTTTVN 
TCav3 NT 5′1 TGATGTTTTATTCAAGTCATGC 
TCav3 NT 5′2 TACTTAGCTAGCCGCGGGAGCCACCATGGATATTTCGTTCGCTATCAT 
TCav3 NT 3′1 CATCAGGCGTCTTAACTTTGC 
TCav3 NT 3′2 TGCTCTGTGGTGGATCTCGAG 
TCav3 CT 3′1 CTTGATATTGATTAAATTTATCCAGATG 
TCav3 CT 3′2 ACTCATCCCGGGTTATACTAATGTTTGAATCAT 
TCav3 CT 5′1 AAATCTGGTAGATCCTAATGAAGTC 
TCav3 CT 5′2 TCGCGTTCCAATTGGTCCTCGAG 
TCav1_F AGTCTTTACCGATTTGGCTCTTCTTTG 
TCav1_R CAATTATACGACTGACACTCTTTCAAAGC 
TCav2_F CGTTTACAAAAGTCTGGATCAAGTTGCG 
TCav2_R CTGCTGTACATTTTGATATGTTAGTAAGATCATCTG 
TCav3_F GTAGAAGATCAGATGGAATTAACGGCTTCC 
TCav3_R TACTAATGTTTGAATCATTTTTCGAGGTAATGTGACC 
TCavβ_F AGTAATAAATTCTCAGGATTAGTGGAAGTGTCC 
TCavβ_R AACATCATTACGTTTTATTACTAGAGGATCTTG 
TCavα2δ-a_F CTCAAGCAGTGGACTAACTAAACTTTACC 
TCavα2δ-a_R CATAGCTGGTTATATGTCACAAATTCCTCTC 
TCavα2δ-b_F TTTACCACATCCTTCTTCAGCTTCTTGAC 
TCavα2δ-b_R AGCATACACTGATAGCTTTACATAGCG 
TCavα2δ-c_F GATATTCGAAATTTCGAATGATACCCTTTGC 
TCavα2δ-c-R CAACGCGAGAAGTAGAAGTGTCCG 

Translation start and stop codons are in bold, and the Kozak sequences and restriction enzyme sites used for cloning are underlined.

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