Table 1. Numbers and densities of... | Rockefeller University Press

Table 1.

Numbers and densities of channels in native inside-out patches from ramp traces recorded from PE (PA14) and EC (Frag1) giant spheroplasts

Cell type	No. of MscS per patch	No. of MscL per patch	Midpoint ratio p_MscS/p_MscL	MscL midpoint	Patch radius	Patch area	MscS/µm²	MscL/µm²	n
				mmHg	µm	µm²
Frag1	62 ± 6 (1 nS)	47 ± 3 (3 nS)	0.58 ± 0.02	156 ± 4	1.3 ± 0.1	11 ± 1	6 ± 1	4.4 ± 0.3	12
PA14	13 ± 4 (0.97 nS)	78 ± 10 (2.1 nS)	0.54 ± 0.02	162 ± 13	1.4 ± 0.2	13 ± 3	1.2 ± 0.4	7 ± 1	8

Cell type	No. of MscS per patch	No. of MscL per patch	Midpoint ratio p_MscS/p_MscL	MscL midpoint	Patch radius	Patch area	MscS/µm²	MscL/µm²	n
				mmHg	µm	µm²
Frag1	62 ± 6 (1 nS)	47 ± 3 (3 nS)	0.58 ± 0.02	156 ± 4	1.3 ± 0.1	11 ± 1	6 ± 1	4.4 ± 0.3	12
PA14	13 ± 4 (0.97 nS)	78 ± 10 (2.1 nS)	0.54 ± 0.02	162 ± 13	1.4 ± 0.2	13 ± 3	1.2 ± 0.4	7 ± 1	8

A ±15% uncertainty in the absolute values of midpoint tensions for EC and PA MscL may result in an ∼30% error in patch area and a similar error in channel density estimation.

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