| Construct . | Total events (# of patches) . | i . | eq. Po . | MCT . | MOT . |
|---|---|---|---|---|---|
| pA | ms | ms | |||
| N1/N2A | 1,531,680 (10) | −7.2 ± 0.1 | 0.69 ± 0.04 | 2.3 ± 0.2 | 5.5 ± 0.5 |
| N1(N812W) | 147,374 (5) | −4.1 ± 0.5* | 0.03 ± 0.01* | 31.8 ± 10.3* | 0.40 ± 0.07* |
| N1(M813W) | 484,988 (4) | −4.4 ± 0.4* | 0.04 ± 0.01* | 7.3 ± 1.5* | 0.28 ± 0.05* |
| N1(V816W) | 475,752 (4) | −5.3 ± 0.7* | 0.02 ± 0.01* | 7.2 ± 2.2 | 0.13 ± 0.01* |
| N1(G827W) | 218,498 (4) | −7.6 ± 0.5 | 0.30 ± 0.05* | 11.6 ± 1.9* | 4.7 ± 0.8 |
| N1(E834W) | 198,394 (4) | −7.0 ± 0.3 | 0.64 ± 0.07 | 3.8 ± 0.8 | 6.5 ± 0.6 |
| N2A(M817W) | 20,985 (5) | −8.0 ± 0.2 | 0.001 ± 0.0005* | 213 ± 73* | 0.20 ± 0.10* |
| N2A(G819W) | 27,892 (5) | −5.4 ± 0.3* | 0.005 ± 0.001* | 73.8 ± 7.1* | 0.33 ± 0.03* |
| N2A(E838W) | 81,550 (4) | −6.6 ± 0.4 | 0.54 ± 0.08 | 9.1 ± 3.4 | 8.8 ± 0.6* |
| Construct . | Total events (# of patches) . | i . | eq. Po . | MCT . | MOT . |
|---|---|---|---|---|---|
| pA | ms | ms | |||
| N1/N2A | 1,531,680 (10) | −7.2 ± 0.1 | 0.69 ± 0.04 | 2.3 ± 0.2 | 5.5 ± 0.5 |
| N1(N812W) | 147,374 (5) | −4.1 ± 0.5* | 0.03 ± 0.01* | 31.8 ± 10.3* | 0.40 ± 0.07* |
| N1(M813W) | 484,988 (4) | −4.4 ± 0.4* | 0.04 ± 0.01* | 7.3 ± 1.5* | 0.28 ± 0.05* |
| N1(V816W) | 475,752 (4) | −5.3 ± 0.7* | 0.02 ± 0.01* | 7.2 ± 2.2 | 0.13 ± 0.01* |
| N1(G827W) | 218,498 (4) | −7.6 ± 0.5 | 0.30 ± 0.05* | 11.6 ± 1.9* | 4.7 ± 0.8 |
| N1(E834W) | 198,394 (4) | −7.0 ± 0.3 | 0.64 ± 0.07 | 3.8 ± 0.8 | 6.5 ± 0.6 |
| N2A(M817W) | 20,985 (5) | −8.0 ± 0.2 | 0.001 ± 0.0005* | 213 ± 73* | 0.20 ± 0.10* |
| N2A(G819W) | 27,892 (5) | −5.4 ± 0.3* | 0.005 ± 0.001* | 73.8 ± 7.1* | 0.33 ± 0.03* |
| N2A(E838W) | 81,550 (4) | −6.6 ± 0.4 | 0.54 ± 0.08 | 9.1 ± 3.4 | 8.8 ± 0.6* |
Values shown are mean ± SEM for single-channel current amplitude (i), equilibrium open probability (eq. Po), MCT, and MOT. Single-channel currents were recorded in the on-cell mode at approximately −100 mV and analyzed in QuB (see Materials and methods). Number of patches is in parenthesis to the right of total events. Eq. Po is the fractional occupancy of the open states in the entire single-channel recording, including long-lived closed states. All data were idealized and fit at a dead time of 40 µs. Asterisks indicate values significantly different from wild type (P < 0.05, t test).