| Mutants . | EC50 ± SEM Hill slope . | |
|---|---|---|
| µM | ||
| WT | 40.5 ± 4.5 2.6 | |
| H1346A | NF NA | |
| H1346W | 112.5 ± 6.4 2.0 | |
| T1347A | NF NA | |
| T1347F | 92.9 ± 11.2 1.2 | |
| T1347Y | 115.1 ± 13.4 1.3 | |
| Y1349A | NF NA | |
| Y1349I | >10 mM NA | |
| L1379A | ND NA | |
| L1379S | 10.8 ± 3.5 1.0 | |
| S1391A | 3.5 ± 0.2 2.7 | |
| E1409A | 506.3 ± 108.7 1.5 | |
| E1409Q | 5.4 ± 1.6 1.6 | |
| D1431A | NF NA | |
| D1431N | >100 mM NA | |
| R1433A | NF NA | |
| R1433K | NF NA | |
| R1433G | >10 mM NA | |
| L1484V | ND NA | |
| H1488A | NF NA | |
| H1488F | >10 mM NA | |
| Mutants . | EC50 ± SEM Hill slope . | |
|---|---|---|
| µM | ||
| WT | 40.5 ± 4.5 2.6 | |
| H1346A | NF NA | |
| H1346W | 112.5 ± 6.4 2.0 | |
| T1347A | NF NA | |
| T1347F | 92.9 ± 11.2 1.2 | |
| T1347Y | 115.1 ± 13.4 1.3 | |
| Y1349A | NF NA | |
| Y1349I | >10 mM NA | |
| L1379A | ND NA | |
| L1379S | 10.8 ± 3.5 1.0 | |
| S1391A | 3.5 ± 0.2 2.7 | |
| E1409A | 506.3 ± 108.7 1.5 | |
| E1409Q | 5.4 ± 1.6 1.6 | |
| D1431A | NF NA | |
| D1431N | >100 mM NA | |
| R1433A | NF NA | |
| R1433K | NF NA | |
| R1433G | >10 mM NA | |
| L1484V | ND NA | |
| H1488A | NF NA | |
| H1488F | >10 mM NA | |
NA, not applicable; ND, EC50 value is not significantly different from the WT, based on the measurements of currents induced by EC10 and EC90 concentrations of ADPR; NF, EC50 value is not defined because the mutation fails to yield a saturated response even at millimolar concentrations of ADPR.