Table 1. Cl − dependence of the activity... | Rockefeller University Press

Table 1.

Cl⁻ dependence of the activity of CLC-ec1 measured by H⁺ transport

Protein	[Cl⁻]	[H]/[H]_300Cl	n
	mM
WT CLC-ec1	0	0.013 ± 0.001	3
	50	0.30 ± 0.03	7
	70	0.46 ± 0.02	17
	100	0.76 ± 0.03	3
	150	0.86 ± 0.01	3
	300	1.00 ± 0.02	29
	500	0.90 ± 0.04	23
	750	0.73 ± 0.06	14
	1,000	0.35 ± 0.03	16
S107A	70	0.31 ± 0.01	8
	300	1.00 ± 0.03	11
	500	1.57 ± 0.37	11
	750	0.89 ± 0.12	11
	1,000	0.27 ± 0.03	8
Y445F	70	0.44 ± 0.02	9
	300	1.00 ± 0.06	9
	500	1.36 ± 0.06	10
	750	0.73 ± 0.09	10
	1,000	0.27 ± 0.06	8
S107A/Y445F	0	0.012 ± 0.001	3
	70	0.63 ± 0.06	11
	300	1.00 ± 0.05	8
	500	1.13 ± 0.11	11
	750	1.07 ± 0.13	11
	1,000	0.40 ± 0.05	10

Protein	[Cl⁻]	[H]/[H]_300Cl	n
	mM
WT CLC-ec1	0	0.013 ± 0.001	3
	50	0.30 ± 0.03	7
	70	0.46 ± 0.02	17
	100	0.76 ± 0.03	3
	150	0.86 ± 0.01	3
	300	1.00 ± 0.02	29
	500	0.90 ± 0.04	23
	750	0.73 ± 0.06	14
	1,000	0.35 ± 0.03	16
S107A	70	0.31 ± 0.01	8
	300	1.00 ± 0.03	11
	500	1.57 ± 0.37	11
	750	0.89 ± 0.12	11
	1,000	0.27 ± 0.03	8
Y445F	70	0.44 ± 0.02	9
	300	1.00 ± 0.06	9
	500	1.36 ± 0.06	10
	750	0.73 ± 0.09	10
	1,000	0.27 ± 0.06	8
S107A/Y445F	0	0.012 ± 0.001	3
	70	0.63 ± 0.06	11
	300	1.00 ± 0.05	8
	500	1.13 ± 0.11	11
	750	1.07 ± 0.13	11
	1,000	0.40 ± 0.05	10

Data are reported as the mean ± SEM of n experiments from two to eight independent preparations.