Table 2.
[3H]Ouabain-bound and maximal turnover rates of outward current produced by wild-type pumps with 3 mM K+o in NMG+o (t/oout) or of inward currents produced by mutant pumps (t/oin) in Na+o without K+o, measured at −50 mV
Mutantn[3H]Ouabaint/oout (s−1)t/oin (s−1)
3 K+oNa+o
  fmol    
Wild type 17 146 ± 13 32.0 ± 1.5   
L104R 14 317 ± 32   22.8 ± 1.9 
V332G 12 220 ± 9   63.4 ± 6.2 
delF100-L104 62.4 ± 4.7   522 ± 120 
EETA963S 10 314 ± 19   19.7 ± 1.5 
Mutantn[3H]Ouabaint/oout (s−1)t/oin (s−1)
3 K+oNa+o
  fmol    
Wild type 17 146 ± 13 32.0 ± 1.5   
L104R 14 317 ± 32   22.8 ± 1.9 
V332G 12 220 ± 9   63.4 ± 6.2 
delF100-L104 62.4 ± 4.7   522 ± 120 
EETA963S 10 314 ± 19   19.7 ± 1.5 

n, number of oocytes.

or Create an Account

Close Modal
Close Modal