Table 2.
[
3
H]Ouabain-bound and maximal turnover rates of outward current produced by wild-type pumps with 3 mM K
+
o
in NMG
+
o
(t/o
out
) or of inward currents produced by mutant pumps (t/o
in
) in Na
+
o
without K
+
o
, measured at −50 mV
Mutant
n
[
3
H]Ouabain
t/o
out
(s
−1
)
t/o
in
(s
−1
)
3 K
+
o
Na
+
o
fmol
Wild type
17
146 ± 13
32.0 ± 1.5
L104R
14
317 ± 32
22.8 ± 1.9
V332G
12
220 ± 9
63.4 ± 6.2
delF100-L104
4
62.4 ± 4.7
522 ± 120
EETA963S
10
314 ± 19
19.7 ± 1.5
Mutant
n
[
3
H]Ouabain
t/o
out
(s
−1
)
t/o
in
(s
−1
)
3 K
+
o
Na
+
o
fmol
Wild type
17
146 ± 13
32.0 ± 1.5
L104R
14
317 ± 32
22.8 ± 1.9
V332G
12
220 ± 9
63.4 ± 6.2
delF100-L104
4
62.4 ± 4.7
522 ± 120
EETA963S
10
314 ± 19
19.7 ± 1.5
n
, number of oocytes.
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