Table 3.
Effects of variation of extracellular [Ca2+] on Ca2+ transients
StrainMyofiber IDVtest1.8 mM Ca2+5.3 mM Ca2+Ratio
Peak F˙Peak F˙
  mV u.a./ms  u.a./ms  
BALB/c 20160907a 20 22.29  28.06 1.26 
 20160908a 20 12.00  15.34 1.28 
 20160908e 40 16.30  17.20 1.05 
 20160908f 20 19.47  24.29 1.25 
     Mean 1.21 
     SEM 0.05 
       
B6D2F1 20160504d 20 40.24  48.43 1.20 
 20160520a 40 5.45  4.64 0.85 
 20160506d 40 4.24  9.12 2.15 
 20160506a 40 7.09  15.64 2.21 
 20160506c 20 7.25  14.22 1.96 
 20160504e 40 65.17  94.35 1.45 
 20160511c 40 22.89  59.42 2.59 
     Mean 1.8 
     SEM 0.24 
StrainMyofiber IDVtest1.8 mM Ca2+5.3 mM Ca2+Ratio
Peak F˙Peak F˙
  mV u.a./ms  u.a./ms  
BALB/c 20160907a 20 22.29  28.06 1.26 
 20160908a 20 12.00  15.34 1.28 
 20160908e 40 16.30  17.20 1.05 
 20160908f 20 19.47  24.29 1.25 
     Mean 1.21 
     SEM 0.05 
       
B6D2F1 20160504d 20 40.24  48.43 1.20 
 20160520a 40 5.45  4.64 0.85 
 20160506d 40 4.24  9.12 2.15 
 20160506a 40 7.09  15.64 2.21 
 20160506c 20 7.25  14.22 1.96 
 20160504e 40 65.17  94.35 1.45 
 20160511c 40 22.89  59.42 2.59 
     Mean 1.8 
     SEM 0.24 

Fluorescence signals were recorded in response to large depolarizing pulses in reference 1.8 mM external [Ca2+] and after changing external [Ca2+] to 5.3 mM to keep total divalent cation concentration constant. [Mg2+] was 3.5 mM in the reference solution and 0 mM in the high-[Ca2+] solution. In both strains, the differences in peak amplitude in the two conditions were significantly different (P < 0.05).

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