| Strain . | Myofiber ID . | Vtest . | 1.8 mM Ca2+ . | . | 5.3 mM Ca2+ . | Ratio . |
|---|---|---|---|---|---|---|
| Peak . | Peak . | |||||
| mV | u.a./ms | u.a./ms | ||||
| BALB/c | 20160907a | 20 | 22.29 | 28.06 | 1.26 | |
| 20160908a | 20 | 12.00 | 15.34 | 1.28 | ||
| 20160908e | 40 | 16.30 | 17.20 | 1.05 | ||
| 20160908f | 20 | 19.47 | 24.29 | 1.25 | ||
| Mean | 1.21 | |||||
| SEM | 0.05 | |||||
| B6D2F1 | 20160504d | 20 | 40.24 | 48.43 | 1.20 | |
| 20160520a | 40 | 5.45 | 4.64 | 0.85 | ||
| 20160506d | 40 | 4.24 | 9.12 | 2.15 | ||
| 20160506a | 40 | 7.09 | 15.64 | 2.21 | ||
| 20160506c | 20 | 7.25 | 14.22 | 1.96 | ||
| 20160504e | 40 | 65.17 | 94.35 | 1.45 | ||
| 20160511c | 40 | 22.89 | 59.42 | 2.59 | ||
| Mean | 1.8 | |||||
| SEM | 0.24 |
| Strain . | Myofiber ID . | Vtest . | 1.8 mM Ca2+ . | . | 5.3 mM Ca2+ . | Ratio . |
|---|---|---|---|---|---|---|
| Peak . | Peak . | |||||
| mV | u.a./ms | u.a./ms | ||||
| BALB/c | 20160907a | 20 | 22.29 | 28.06 | 1.26 | |
| 20160908a | 20 | 12.00 | 15.34 | 1.28 | ||
| 20160908e | 40 | 16.30 | 17.20 | 1.05 | ||
| 20160908f | 20 | 19.47 | 24.29 | 1.25 | ||
| Mean | 1.21 | |||||
| SEM | 0.05 | |||||
| B6D2F1 | 20160504d | 20 | 40.24 | 48.43 | 1.20 | |
| 20160520a | 40 | 5.45 | 4.64 | 0.85 | ||
| 20160506d | 40 | 4.24 | 9.12 | 2.15 | ||
| 20160506a | 40 | 7.09 | 15.64 | 2.21 | ||
| 20160506c | 20 | 7.25 | 14.22 | 1.96 | ||
| 20160504e | 40 | 65.17 | 94.35 | 1.45 | ||
| 20160511c | 40 | 22.89 | 59.42 | 2.59 | ||
| Mean | 1.8 | |||||
| SEM | 0.24 |
Fluorescence signals were recorded in response to large depolarizing pulses in reference 1.8 mM external [Ca2+] and after changing external [Ca2+] to 5.3 mM to keep total divalent cation concentration constant. [Mg2+] was 3.5 mM in the reference solution and 0 mM in the high-[Ca2+] solution. In both strains, the differences in peak amplitude in the two conditions were significantly different (P < 0.05).