Table 3.
Buffer effects of Na+-binding enthalpy
BufferBuffer pH−ΔH(H+)aKd(Na+) (mM)ΔG(Na+)ΔHITC(Na+)Slope
  kJ/mol mM kJ/mol kJ/mol  
KPi 6.45 (nb = 2) 3.6 1.16 ± 0.04c −16.76 ± 0.08 −38.22 ± 0.67  
MES-TMAOH 6.25 (n = 3) 14.8 0.90 ± 0.01 −17.38 ± 0.02 −45.70 ± 0.28 −0.48 ± 0.09 
ACES-TMAOH 6.25 (n = 2) 30.43 0.99 ± 0.06 −17.16 ± 0.16 −51.37 ± 0.32  
KPi 7.45 (n = 3) 3.6 0.65 ± 0.02 −18.19 ± 0.09 −21.67 ± 0.34  
HEPES-TMAOH 7.45 (n = 3) 20.3 0.52 ± 0.03 −18.75 ± 0.12 −24.79 ± 0.23 −0.21 ± 0.03 
Tris-HCl 7.45 (n = 4) 47.4 0.64 ± 0.02 −18.24 ± 0.08 −31.50 ± 0.63  
KPi 8.2 (n = 1) 3.6 0.72 −17.94 -18.06  
HEPES-TMAOH 8.2 (n = 2) 20.3 0.56 ± 0.01 −18.55 ± 0.11 −18.93 ± 0.12 −0.04 ± 0.01 
Tris-HCl 8.2 (n = 2) 47.4 0.50 ± 0.01 −18.90 ± 0.05 −19.76 ± 0.26  
BufferBuffer pH−ΔH(H+)aKd(Na+) (mM)ΔG(Na+)ΔHITC(Na+)Slope
  kJ/mol mM kJ/mol kJ/mol  
KPi 6.45 (nb = 2) 3.6 1.16 ± 0.04c −16.76 ± 0.08 −38.22 ± 0.67  
MES-TMAOH 6.25 (n = 3) 14.8 0.90 ± 0.01 −17.38 ± 0.02 −45.70 ± 0.28 −0.48 ± 0.09 
ACES-TMAOH 6.25 (n = 2) 30.43 0.99 ± 0.06 −17.16 ± 0.16 −51.37 ± 0.32  
KPi 7.45 (n = 3) 3.6 0.65 ± 0.02 −18.19 ± 0.09 −21.67 ± 0.34  
HEPES-TMAOH 7.45 (n = 3) 20.3 0.52 ± 0.03 −18.75 ± 0.12 −24.79 ± 0.23 −0.21 ± 0.03 
Tris-HCl 7.45 (n = 4) 47.4 0.64 ± 0.02 −18.24 ± 0.08 −31.50 ± 0.63  
KPi 8.2 (n = 1) 3.6 0.72 −17.94 -18.06  
HEPES-TMAOH 8.2 (n = 2) 20.3 0.56 ± 0.01 −18.55 ± 0.11 −18.93 ± 0.12 −0.04 ± 0.01 
Tris-HCl 8.2 (n = 2) 47.4 0.50 ± 0.01 −18.90 ± 0.05 −19.76 ± 0.26  
a

Standards for the buffer protonation enthalpy (Goldberg et al., 2002).

b

n = number of tests.

c

Standard error (SEM).

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