Table 2.
Absolute dissociation constants for Na
+
or H
+
binding to MelB
St
Parameter
.
No sugar
.
With melibiose
.
Absolute
K
D(Na+)
(mM)
a
0.54 ± 0.03
b
0.09 ± 0.02
Absolute
K
D(H+)
(μM)
0.56 ± 0.03
0.26 ± 0.02
Cation selectivity ratio (slope =
K
D(Na+)
/
K
D(H+)
)
964
346
p
K
a
6.25
6.59
Parameter
.
No sugar
.
With melibiose
.
Absolute
K
D(Na+)
(mM)
a
0.54 ± 0.03
b
0.09 ± 0.02
Absolute
K
D(H+)
(μM)
0.56 ± 0.03
0.26 ± 0.02
Cation selectivity ratio (slope =
K
D(Na+)
/
K
D(H+)
)
964
346
p
K
a
6.25
6.59
a
Data were extracted from Fig. 4.
b
Standard error from curve fitting.
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