Table 1. Specific primer pairs used for... | Rockefeller University Press

Table 1.

Specific primer pairs used for PCR amplification

Target	Forward primer 5′-3′	Reverse primer 5′-3′	Size
			bp
P2X1	GAGAGTCGGGCCAGGACTTC	GCGAATCCCAAACACCTTGA	233
P2X2	TCCCTCCCCCACCTAGTCAC	CACCACCTGCTCAGTCAGAGC	149
P2X3	CTGCCTAACCTCACCGACAAG	AATACCCAGAACGCCACCC	150
P2X4	CCCTTTGCCTGCCCAGATAT	CCGTACGCCTTGGTGAGTGT	145
P2X5	GGAAGATAATGTTGAGGTTGA	TCCTGACGAACCCTCTCCAGT	81
P2X5b	GCTGCCTCCCACTGCAACCC	AAGCCCCAGCACCCATGAGC	253
P2X6	TCCAGAGCATCCTTCCGTTCC	GGCACCAGCTCCAGATCTCA	152
P2X7	GCACGAATTATGGCACCGTC	CCCCACCCTCTGTGACATTCT	171
P2X2_splice	GACCTCCATCGGGGTGGGCT	ATGTCCTGGGAGCCGAAGCG	116
P2X7_splice	GTCTCGCCTACCGGAGCAACG	TGGGGTCCGTGGATGTGGAGT	237
β-actin	GTCTTCCCCTCCATCGTGG	TGGATGCCACAGGATTCC	750

Target	Forward primer 5′-3′	Reverse primer 5′-3′	Size
			bp
P2X1	GAGAGTCGGGCCAGGACTTC	GCGAATCCCAAACACCTTGA	233
P2X2	TCCCTCCCCCACCTAGTCAC	CACCACCTGCTCAGTCAGAGC	149
P2X3	CTGCCTAACCTCACCGACAAG	AATACCCAGAACGCCACCC	150
P2X4	CCCTTTGCCTGCCCAGATAT	CCGTACGCCTTGGTGAGTGT	145
P2X5	GGAAGATAATGTTGAGGTTGA	TCCTGACGAACCCTCTCCAGT	81
P2X5b	GCTGCCTCCCACTGCAACCC	AAGCCCCAGCACCCATGAGC	253
P2X6	TCCAGAGCATCCTTCCGTTCC	GGCACCAGCTCCAGATCTCA	152
P2X7	GCACGAATTATGGCACCGTC	CCCCACCCTCTGTGACATTCT	171
P2X2_splice	GACCTCCATCGGGGTGGGCT	ATGTCCTGGGAGCCGAAGCG	116
P2X7_splice	GTCTCGCCTACCGGAGCAACG	TGGGGTCCGTGGATGTGGAGT	237
β-actin	GTCTTCCCCTCCATCGTGG	TGGATGCCACAGGATTCC	750