| . | Divalent solutions . | Monovalent solutions . |
|---|---|---|
| mM | mM | |
| NMDG | 120 | 22 |
| HEPES | 20 | 20 |
| Permeant ion | 2 | 100 |
| . | Divalent solutions . | Monovalent solutions . |
|---|---|---|
| mM | mM | |
| NMDG | 120 | 22 |
| HEPES | 20 | 20 |
| Permeant ion | 2 | 100 |
For divalent solutions, the permeant ions were Mg2+, Ca2+, or Ba2+. For monovalent solutions, the permeant ions were Li+, Na+, or K+. The pH was adjusted to 7.4 using methane-thiosulfonic acid. The osmolarity of all solutions was 245–260 mosmol.