Table 2.
Composition of media
MediaNa+K+Cl−Ca2+Mg2+SO2−4NO−3HCO−3SiHEPNMGGLUpHOsM
B1 96 1.0 85 0.8 0.8 0.8 0.7 2.5 0.0 10.0 1.0 1.0 7.4 200 
B2 96 1.0 85 0.8 0.8 0.8 0.7 2.5 2.0 10.0 0.0 0.0 7.4 200 
B3 88 7.0 89 2.0 2.0 1.0 0.0 0.0 0.0 10.0 0.0 0.0 7.4 200 
MediaNa+K+Cl−Ca2+Mg2+SO2−4NO−3HCO−3SiHEPNMGGLUpHOsM
B1 96 1.0 85 0.8 0.8 0.8 0.7 2.5 0.0 10.0 1.0 1.0 7.4 200 
B2 96 1.0 85 0.8 0.8 0.8 0.7 2.5 2.0 10.0 0.0 0.0 7.4 200 
B3 88 7.0 89 2.0 2.0 1.0 0.0 0.0 0.0 10.0 0.0 0.0 7.4 200 

Abbreviations are HEP = HEPES, NMG = N-methyl-d-glucamine, GLU = gluconate, OsM = osmolality. Except for pH and OsM, all units are in mM.

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