Table 1.
Mutants generated and oligonucleotides used
NameMutagenic oligonucleotides
AQP1L84C                              5′ gtcacactggggctgTGCctTagctgccagatcag 
                              3′ ctgatctggcagctAagGCAcagccccagtgtgac 
AQP1H180G                              5′ ctctctgtagcccttggaGGCctcctggctattgacAAC 
                              3′ GTTgtcaatagccaggagGCCtccaagggctacagagag 
AQP1F56G/H180G                              5′ gaaggtgtcgctggccGGCgggctgagcatcg 
                              3′ cgatgctcagcccGCCggccagcgacaccttc 
AQP1Y186N                              5′ ggaggCctcctggcAattgacAACactggctgtggg 
                              3′ cccacagccagtGTTgtcaatTgccaggagGcctcc 
AQP1LGRND↔IFATY                              5′ ctgactgggaactcgATTTTTGCCACCTATctggctgatggtgtg 
                              3′ cacaccatcagccagATAGGTGGCAAAAATcgagttcccagtcag 
AQP10C90L                              5′ ccttctccctggccatgCTCatcgttggacgcctccc 
                              3′ gggaggcgtccaacgatGAGcatggccagggagaagg 
AQP10G202H                              5′ ctgatcctggccctcCACttatcTatgggtgcc 
                              3′ ggcacccatAgataaGTGgagggccaggatcag 
AQP10G62F/G202H                              5′ gtttctggctTTctctctggccgttacgatCgccatctacgtg 
                              3′ cacgtagatggcGatcgtaacggccagagagAAagccagaaac 
AQP10N208Y                              5′ ggccctcgggttatcTatgggtgccTACtgcgggattccactc 
                              3′ gagtggaatcccgcaGTAggcacccatAgataacccgagggcc 
AQP10IFATY↔LGRND                              5′ aaggagacagcctccCTTGGCCGCAATGACcctgccccctatctg 
                              3′ cagatagggggcaggGTCATTGCGGCCAAGggaggctgtctcctt 
+PmlI in AQP1                              5′ atcagcggcgcccacgtgAACCCGGCTgtcac 
                              3′ gtgacAGCCGGGTTcacgtgggcgccgctgat 
+SacII in AQP1                              5′ tgtgggattAACCCTCCGcggtcctttggctcc 
                              3′ ggagccaaaggaccgCGGAGGGTTaatcccaca 
+Pml1 in AQP10                              5′ gtctcaggggcccacgtgAATCCAGCCttc 
                              3′ gaaGGCTGGATTcacgtgggcccctgagac 
+SacII in AQP10                              5′ gtctcaggggcccacgtgAATCCAGCCttc 
                              3′ tgggcccaggtcccgCGGAGGGTTgagtggaat 
5′ AQP1-10-1                              5′ atcagcggcgcccacctcAATCCAGCCttctcc 
                              3′ ggagaaGGCTGGATTgaggtgggcgccgctgat 
3′ AQP1-10-1                              5′ attccactcAACCCTGCCcggtcctttggctcc 
                              3′ ggagccaaaggaccgGGCAGGGTTgagtggaat 
5′ AQP10-1-10                              5′ gtctcaggggcccacctgAACCCGGCTgtcac 
                              3′ gtgacAGCCGGGTTcaggtgggcccctgagac 
3′ AQP10-1-10                              5′ tgtgggattAACCCTGCTcgggacctgggccca 
                              3′ tgggcccaggtcccgAGCAGGGTTaatcccaca 
NameMutagenic oligonucleotides
AQP1L84C                              5′ gtcacactggggctgTGCctTagctgccagatcag 
                              3′ ctgatctggcagctAagGCAcagccccagtgtgac 
AQP1H180G                              5′ ctctctgtagcccttggaGGCctcctggctattgacAAC 
                              3′ GTTgtcaatagccaggagGCCtccaagggctacagagag 
AQP1F56G/H180G                              5′ gaaggtgtcgctggccGGCgggctgagcatcg 
                              3′ cgatgctcagcccGCCggccagcgacaccttc 
AQP1Y186N                              5′ ggaggCctcctggcAattgacAACactggctgtggg 
                              3′ cccacagccagtGTTgtcaatTgccaggagGcctcc 
AQP1LGRND↔IFATY                              5′ ctgactgggaactcgATTTTTGCCACCTATctggctgatggtgtg 
                              3′ cacaccatcagccagATAGGTGGCAAAAATcgagttcccagtcag 
AQP10C90L                              5′ ccttctccctggccatgCTCatcgttggacgcctccc 
                              3′ gggaggcgtccaacgatGAGcatggccagggagaagg 
AQP10G202H                              5′ ctgatcctggccctcCACttatcTatgggtgcc 
                              3′ ggcacccatAgataaGTGgagggccaggatcag 
AQP10G62F/G202H                              5′ gtttctggctTTctctctggccgttacgatCgccatctacgtg 
                              3′ cacgtagatggcGatcgtaacggccagagagAAagccagaaac 
AQP10N208Y                              5′ ggccctcgggttatcTatgggtgccTACtgcgggattccactc 
                              3′ gagtggaatcccgcaGTAggcacccatAgataacccgagggcc 
AQP10IFATY↔LGRND                              5′ aaggagacagcctccCTTGGCCGCAATGACcctgccccctatctg 
                              3′ cagatagggggcaggGTCATTGCGGCCAAGggaggctgtctcctt 
+PmlI in AQP1                              5′ atcagcggcgcccacgtgAACCCGGCTgtcac 
                              3′ gtgacAGCCGGGTTcacgtgggcgccgctgat 
+SacII in AQP1                              5′ tgtgggattAACCCTCCGcggtcctttggctcc 
                              3′ ggagccaaaggaccgCGGAGGGTTaatcccaca 
+Pml1 in AQP10                              5′ gtctcaggggcccacgtgAATCCAGCCttc 
                              3′ gaaGGCTGGATTcacgtgggcccctgagac 
+SacII in AQP10                              5′ gtctcaggggcccacgtgAATCCAGCCttc 
                              3′ tgggcccaggtcccgCGGAGGGTTgagtggaat 
5′ AQP1-10-1                              5′ atcagcggcgcccacctcAATCCAGCCttctcc 
                              3′ ggagaaGGCTGGATTgaggtgggcgccgctgat 
3′ AQP1-10-1                              5′ attccactcAACCCTGCCcggtcctttggctcc 
                              3′ ggagccaaaggaccgGGCAGGGTTgagtggaat 
5′ AQP10-1-10                              5′ gtctcaggggcccacctgAACCCGGCTgtcac 
                              3′ gtgacAGCCGGGTTcaggtgggcccctgagac 
3′ AQP10-1-10                              5′ tgtgggattAACCCTGCTcgggacctgggccca 
                              3′ tgggcccaggtcccgAGCAGGGTTaatcccaca 

Uppercase letters correspond to a base pair that was mutated to generate a residue substitution, to remove a restriction site or to add a restriction site.

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