Table 1. Mutants generated and... | Rockefeller University Press

Table 1.

Mutants generated and oligonucleotides used

Name	Mutagenic oligonucleotides
AQP1_L84C	5′ gtcacactggggctgTGCctTagctgccagatcag
	3′ ctgatctggcagctAagGCAcagccccagtgtgac
AQP1_H180G	5′ ctctctgtagcccttggaGGCctcctggctattgacAAC
	3′ GTTgtcaatagccaggagGCCtccaagggctacagagag
AQP1_F56G/H180G	5′ gaaggtgtcgctggccGGCgggctgagcatcg
	3′ cgatgctcagcccGCCggccagcgacaccttc
AQP1_Y186N	5′ ggaggCctcctggcAattgacAACactggctgtggg
	3′ cccacagccagtGTTgtcaatTgccaggagGcctcc
AQP1_{LGRND↔IFATY}	5′ ctgactgggaactcgATTTTTGCCACCTATctggctgatggtgtg
	3′ cacaccatcagccagATAGGTGGCAAAAATcgagttcccagtcag
AQP10_C90L	5′ ccttctccctggccatgCTCatcgttggacgcctccc
	3′ gggaggcgtccaacgatGAGcatggccagggagaagg
AQP10_G202H	5′ ctgatcctggccctcCACttatcTatgggtgcc
	3′ ggcacccatAgataaGTGgagggccaggatcag
AQP10_G62F/G202H	5′ gtttctggctTTctctctggccgttacgatCgccatctacgtg
	3′ cacgtagatggcGatcgtaacggccagagagAAagccagaaac
AQP10_N208Y	5′ ggccctcgggttatcTatgggtgccTACtgcgggattccactc
	3′ gagtggaatcccgcaGTAggcacccatAgataacccgagggcc
AQP10_{IFATY↔LGRND}	5′ aaggagacagcctccCTTGGCCGCAATGACcctgccccctatctg
	3′ cagatagggggcaggGTCATTGCGGCCAAGggaggctgtctcctt
+PmlI in AQP1	5′ atcagcggcgcccacgtgAACCCGGCTgtcac
	3′ gtgacAGCCGGGTTcacgtgggcgccgctgat
+SacII in AQP1	5′ tgtgggattAACCCTCCGcggtcctttggctcc
	3′ ggagccaaaggaccgCGGAGGGTTaatcccaca
+Pml1 in AQP10	5′ gtctcaggggcccacgtgAATCCAGCCttc
	3′ gaaGGCTGGATTcacgtgggcccctgagac
+SacII in AQP10	5′ gtctcaggggcccacgtgAATCCAGCCttc
	3′ tgggcccaggtcccgCGGAGGGTTgagtggaat
5′ AQP_1-10-1	5′ atcagcggcgcccacctcAATCCAGCCttctcc
	3′ ggagaaGGCTGGATTgaggtgggcgccgctgat
3′ AQP_1-10-1	5′ attccactcAACCCTGCCcggtcctttggctcc
	3′ ggagccaaaggaccgGGCAGGGTTgagtggaat
5′ AQP_10-1-10	5′ gtctcaggggcccacctgAACCCGGCTgtcac
	3′ gtgacAGCCGGGTTcaggtgggcccctgagac
3′ AQP_10-1-10	5′ tgtgggattAACCCTGCTcgggacctgggccca
	3′ tgggcccaggtcccgAGCAGGGTTaatcccaca

Name	Mutagenic oligonucleotides
AQP1_L84C	5′ gtcacactggggctgTGCctTagctgccagatcag
	3′ ctgatctggcagctAagGCAcagccccagtgtgac
AQP1_H180G	5′ ctctctgtagcccttggaGGCctcctggctattgacAAC
	3′ GTTgtcaatagccaggagGCCtccaagggctacagagag
AQP1_F56G/H180G	5′ gaaggtgtcgctggccGGCgggctgagcatcg
	3′ cgatgctcagcccGCCggccagcgacaccttc
AQP1_Y186N	5′ ggaggCctcctggcAattgacAACactggctgtggg
	3′ cccacagccagtGTTgtcaatTgccaggagGcctcc
AQP1_{LGRND↔IFATY}	5′ ctgactgggaactcgATTTTTGCCACCTATctggctgatggtgtg
	3′ cacaccatcagccagATAGGTGGCAAAAATcgagttcccagtcag
AQP10_C90L	5′ ccttctccctggccatgCTCatcgttggacgcctccc
	3′ gggaggcgtccaacgatGAGcatggccagggagaagg
AQP10_G202H	5′ ctgatcctggccctcCACttatcTatgggtgcc
	3′ ggcacccatAgataaGTGgagggccaggatcag
AQP10_G62F/G202H	5′ gtttctggctTTctctctggccgttacgatCgccatctacgtg
	3′ cacgtagatggcGatcgtaacggccagagagAAagccagaaac
AQP10_N208Y	5′ ggccctcgggttatcTatgggtgccTACtgcgggattccactc
	3′ gagtggaatcccgcaGTAggcacccatAgataacccgagggcc
AQP10_{IFATY↔LGRND}	5′ aaggagacagcctccCTTGGCCGCAATGACcctgccccctatctg
	3′ cagatagggggcaggGTCATTGCGGCCAAGggaggctgtctcctt
+PmlI in AQP1	5′ atcagcggcgcccacgtgAACCCGGCTgtcac
	3′ gtgacAGCCGGGTTcacgtgggcgccgctgat
+SacII in AQP1	5′ tgtgggattAACCCTCCGcggtcctttggctcc
	3′ ggagccaaaggaccgCGGAGGGTTaatcccaca
+Pml1 in AQP10	5′ gtctcaggggcccacgtgAATCCAGCCttc
	3′ gaaGGCTGGATTcacgtgggcccctgagac
+SacII in AQP10	5′ gtctcaggggcccacgtgAATCCAGCCttc
	3′ tgggcccaggtcccgCGGAGGGTTgagtggaat
5′ AQP_1-10-1	5′ atcagcggcgcccacctcAATCCAGCCttctcc
	3′ ggagaaGGCTGGATTgaggtgggcgccgctgat
3′ AQP_1-10-1	5′ attccactcAACCCTGCCcggtcctttggctcc
	3′ ggagccaaaggaccgGGCAGGGTTgagtggaat
5′ AQP_10-1-10	5′ gtctcaggggcccacctgAACCCGGCTgtcac
	3′ gtgacAGCCGGGTTcaggtgggcccctgagac
3′ AQP_10-1-10	5′ tgtgggattAACCCTGCTcgggacctgggccca
	3′ tgggcccaggtcccgAGCAGGGTTaatcccaca

Uppercase letters correspond to a base pair that was mutated to generate a residue substitution, to remove a restriction site or to add a restriction site.

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