Table 3. Changes in H V 1 properties in... | Rockefeller University Press

Table 3.

Changes in H_V1 properties in S1 (101–125) and S1–S2 linker (126–133) mutants versus WT channels

Mutant	Species	Expr. system	I?	τ_act	τ_tail	ΔV_thr	ΔpH slope	Selectivity	Other	Reference
Q102C	CiH_V1 [H150C]	Xenopus i-o	Yes						MTS_i access	Mony et al., 2015
V103C	CiH_V1 [V151C]	Xenopus i-o	Yes						MTS_i open> closed	Mony et al., 2015
I105C	CiH_V1 [I153C]	Xenopus i-o	Yes						MTS_i open> closed	Mony et al., 2015
I106C	CiH_V1 [I154C]	Xenopus i-o	Yes						MTS_i open> closed	Mony et al., 2015
C107A	hH_V1	HEK wc	Yes			−17				Ramsey et al., 2010
C107S	hH_V1								90% dimer	Li et al., 2015
V109C	CiH_V1 [V157C]	Xenopus i-o	Yes						MTS_i open> closed	Mony et al., 2015
V109A	hH_V1	Xenopus i-o	Yes			−11				Hong et al., 2014
D112A	hH_V1	HEK wc	Yes			59	38			Ramsey et al., 2010
D112A^{^a}	hH_V1	COS/HEK wc	Yes	2.2	3.0	41^{^b}	43	Cl^–		Musset et al., 2011
D112A	hH_V1	Vesicle flux							Slows H⁺ flux	Letts, 2014
D112A	hH_V1	Xenopus i-o	No							Hong et al., 2014
D112A	CiH_V1 [D160A]	Xenopus TEVC	No							Chamberlin et al., 2015
D112A	kH_V1 [D51A]	COS/HEK wc	Yes					Cl^–		Smith et al., 2011
D112A	NpHv1 [D66A]	HEK wc	Yes					Cl^–		Chaves et al., 2016
D112C	CiH_V1 [D160C]	Xenopus TEVC	No							Chamberlin et al., 2015
D112C	NpHv1 [D66C]	HEK wc	No							Chaves et al., 2016
D112C/R211C	CiH_V1 [D160C/ R261C]	Xenopus TEVC	Yes				nc	Na⁺		Chamberlin et al., 2015
D112N	hH_V1	HEK wc	Yes			31	42			Ramsey et al., 2010
D112N^{^a}	hH_V1	COS/HEK wc	Yes	2.4	3.0	23^{^b}	35	Cl^–		Musset et al., 2011
D112N/D185A	hH_V1	HEK wc	Yes			103				Ramsey et al., 2010
D112A	hH_V1	Xenopus i-o	No							Hong et al., 2014
D112E	hH_V1	Xenopus i-o	Yes			−13				Hong et al., 2014
D112E	hH_V1	COS/HEK wc	Yes	0.18	7.4/.085	−11^{^b}	34	H⁺	Biexponential tails	Musset et al., 2011
D112E	hH_V1	Xenopus i-o	Yes			−15				Berger and Isacoff, 2011
D112E	kH_V1 [D51E]	COS/HEK wc	Yes					H⁺		Smith et al., 2011
D112E	NpHv1 [D66E]	HEK wc	Yes					H⁺		Chaves et al., 2016
D112E/I127C	CiH_V1 [D160A/I175C]	Xenopus i-o	Yes	nc	4.0					Mony et al., 2015
D112H	hH_V1	COS/HEK wc	Yes	2.0	0.85	13^{^b}	38	Cl^–		Musset et al., 2011
D112H	kH_V1 [D51H]	COS/HEK wc	Yes					Cl^–		Smith et al., 2011
D112H	NpHv1 [D66H]	HEK wc	Yes					Cl^–		Chaves et al., 2016
D112K^{^a}	hH_V1	COS/HEK wc	Yes	0.8	0.22	46^{^b}	40	Cl^–		Musset et al., 2011
D112S	hH_V1	COS/HEK wc	Yes	2.0	4.1	25^{^b}	38	Cl^–		Musset et al., 2011
D112S	hH_V1	Xenopus i-o	No							Berger and Isacoff, 2011
D112S	hH_V1	Vesicle flux							Slows H⁺ flux	Letts, 2014
D112S	kH_V1 [D51S]	COS/HEK wc	Yes					Cl^–		Smith et al., 2011
D112S	NpHv1 [D66S]	HEK wc	Yes					Cl^–		Chaves et al., 2016
D112S/R211S	hH_V1	Xenopus i-o	Yes			24	44	Gu⁺	At pH 8//8	Berger and Isacoff, 2011
D112F^{^a}	hH_V1	COS/HEK wc	Yes	1.6	0.03	44^{^b}	38	Cl^–		Musset et al., 2011
D112R/R211D	hH_V1	Xenopus i-o	Yes					H⁺		Berger and Isacoff, 2011
D112V	hH_V1	COS/HEK wc	No							Musset et al., 2011
D112L	hH_V1	Vesicle flux							Slows H⁺ flux	Letts, 2014
D112I^{^a}	hH_V1	COS/HEK wc	No							DeCoursey, 2015b
D112Q	hH_V1	Xenopus i-o	No							Hong et al., 2014
D112A/L108D^{^a}	hH_V1	COS/HEK wc	No							Morgan et al., 2013
D112V/V109D^{^a}	hH_V1	COS/HEK wc	No							Morgan et al., 2013
D112A/V109D^{^a}	hH_V1	COS/HEK wc	Yes					Cl^–		Morgan et al., 2013
D112A/V110D^{^a}	hH_V1	COS/HEK wc	No							Morgan et al., 2013
D112A/L111D^{^a}	hH_V1	COS/HEK wc	No							Morgan et al., 2013
D112A/A113D^{^a}	hH_V1	COS/HEK wc	No							Morgan et al., 2013
D112A/L114D^{^a}	hH_V1	COS/HEK wc	No							Morgan et al., 2013
D112A/L115D^{^a}	hH_V1	COS/HEK wc	No							Morgan et al., 2013
D112A/V116D^{^a}	hH_V1	COS/HEK wc	Yes					H⁺		Morgan et al., 2013
D112V/V116D^{^a}	hH_V1	COS/HEK wc	Yes					H⁺		Morgan et al., 2013
D112V/V116E^{^a}	hH_V1	COS/HEK wc	Yes					H⁺		Morgan et al., 2013
D112V/V116S^{^a}	hH_V1	COS/HEK wc	Yes					Cl^–		Morgan et al., 2013
D112V/V116N^{^a}	hH_V1	COS/HEK wc	Yes					Cl^–		Morgan et al., 2013
D112A/L117D^{^a}	hH_V1	COS/HEK wc	No							Morgan et al., 2013
D112A/A118D^{^a}	hH_V1	COS/HEK wc	No							Morgan et al., 2013
D112N/I127C	CiH_V1 [D160N/I175C]	Xenopus i-o	No							Mony et al., 2015
D112N/R211S/I127C	CiH_V1 [D160N/R261S/I175C]	Xenopus i-o	Yes			15				Mony et al., 2015
D112N/R211S/G199C	CiH_V1 [D160N/R261S/G249C]	Xenopus i-o	Yes			44				Mony et al., 2015
D112N/G199C	CiH_V1 [D160N/G249C]	Xenopus i-o	No							Mony et al., 2015
A113D^{^a}	hH_V1	COS/HEK wc	Yes					H⁺		Morgan et al., 2013
E119A	hH_V1	HEK wc	Yes			20	47			Ramsey et al., 2010
E119L	hH_V1	Vesicle flux							nc H⁺ flux	Letts, 2014
E119S	mH_V1 [E115S]	HEK wc	Yes						nc Zn²⁺ inhibition	Takeshita et al., 2014
E119S/D123S	mH_V1 [E115S/D119S]	HEK wc	Yes						Weaker Zn²⁺ inhibition	Takeshita et al., 2014
E119A	CiH_V1 [E167A]	Xenopus TEVC	Yes			4				Chamberlin et al., 2014
E119C/R205C	CiH_V1 [E167C/R255C]	Xenopus TEVC	Yes			−2				Chamberlin et al., 2014
E119C/R208	CiH_V1 [E167C/R258C]	Xenopus TEVC	Yes			−52				Chamberlin et al., 2014
D123A	hH_V1	HEK wc	Yes			20	48			Ramsey et al., 2010
D123S	mH_V1 [D119S]	HEK wc	Yes						nc Zn²⁺ inhibition	Takeshita et al., 2014
D123C	CiH_V1 [D171C]	Xenopus i-o	Yes						MTS_o open> closed	Mony et al., 2015
D123A	CiH_V1 [D171A]	Xenopus TEVC	Yes			72				Chamberlin et al., 2014
D123A/R205N	CiH_V1 [D171A/R255N]	Xenopus TEVC	Yes			11				Chamberlin et al., 2014
K125A	hH_V1	HEK wc	Yes			19	47			Ramsey et al., 2010
K125C	CiH_V1 [K173C]	Xenopus i-o	Yes						MTS_o access	Mony et al., 2015
I127C	CiH_V1 [I175C]	Xenopus i-o	Yes			0				Mony et al., 2015
D130A	hH_V1	HEK wc	Yes			13				Ramsey et al., 2010
K131A	hH_V1	HEK wc	Yes			33				Ramsey et al., 2010

Mutant	Species	Expr. system	I?	τ_act	τ_tail	ΔV_thr	ΔpH slope	Selectivity	Other	Reference
Q102C	CiH_V1 [H150C]	Xenopus i-o	Yes						MTS_i access	Mony et al., 2015
V103C	CiH_V1 [V151C]	Xenopus i-o	Yes						MTS_i open> closed	Mony et al., 2015
I105C	CiH_V1 [I153C]	Xenopus i-o	Yes						MTS_i open> closed	Mony et al., 2015
I106C	CiH_V1 [I154C]	Xenopus i-o	Yes						MTS_i open> closed	Mony et al., 2015
C107A	hH_V1	HEK wc	Yes			−17				Ramsey et al., 2010
C107S	hH_V1								90% dimer	Li et al., 2015
V109C	CiH_V1 [V157C]	Xenopus i-o	Yes						MTS_i open> closed	Mony et al., 2015
V109A	hH_V1	Xenopus i-o	Yes			−11				Hong et al., 2014
D112A	hH_V1	HEK wc	Yes			59	38			Ramsey et al., 2010
D112A^a	hH_V1	COS/HEK wc	Yes	2.2	3.0	41^b	43	Cl^–		Musset et al., 2011
D112A	hH_V1	Vesicle flux							Slows H⁺ flux	Letts, 2014
D112A	hH_V1	Xenopus i-o	No							Hong et al., 2014
D112A	CiH_V1 [D160A]	Xenopus TEVC	No							Chamberlin et al., 2015
D112A	kH_V1 [D51A]	COS/HEK wc	Yes					Cl^–		Smith et al., 2011
D112A	NpHv1 [D66A]	HEK wc	Yes					Cl^–		Chaves et al., 2016
D112C	CiH_V1 [D160C]	Xenopus TEVC	No							Chamberlin et al., 2015
D112C	NpHv1 [D66C]	HEK wc	No							Chaves et al., 2016
D112C/R211C	CiH_V1 [D160C/ R261C]	Xenopus TEVC	Yes				nc	Na⁺		Chamberlin et al., 2015
D112N	hH_V1	HEK wc	Yes			31	42			Ramsey et al., 2010
D112N^a	hH_V1	COS/HEK wc	Yes	2.4	3.0	23^b	35	Cl^–		Musset et al., 2011
D112N/D185A	hH_V1	HEK wc	Yes			103				Ramsey et al., 2010
D112A	hH_V1	Xenopus i-o	No							Hong et al., 2014
D112E	hH_V1	Xenopus i-o	Yes			−13				Hong et al., 2014
D112E	hH_V1	COS/HEK wc	Yes	0.18	7.4/.085	−11^b	34	H⁺	Biexponential tails	Musset et al., 2011
D112E	hH_V1	Xenopus i-o	Yes			−15				Berger and Isacoff, 2011
D112E	kH_V1 [D51E]	COS/HEK wc	Yes					H⁺		Smith et al., 2011
D112E	NpHv1 [D66E]	HEK wc	Yes					H⁺		Chaves et al., 2016
D112E/I127C	CiH_V1 [D160A/I175C]	Xenopus i-o	Yes	nc	4.0					Mony et al., 2015
D112H	hH_V1	COS/HEK wc	Yes	2.0	0.85	13^b	38	Cl^–		Musset et al., 2011
D112H	kH_V1 [D51H]	COS/HEK wc	Yes					Cl^–		Smith et al., 2011
D112H	NpHv1 [D66H]	HEK wc	Yes					Cl^–		Chaves et al., 2016
D112K^a	hH_V1	COS/HEK wc	Yes	0.8	0.22	46^b	40	Cl^–		Musset et al., 2011
D112S	hH_V1	COS/HEK wc	Yes	2.0	4.1	25^b	38	Cl^–		Musset et al., 2011
D112S	hH_V1	Xenopus i-o	No							Berger and Isacoff, 2011
D112S	hH_V1	Vesicle flux							Slows H⁺ flux	Letts, 2014
D112S	kH_V1 [D51S]	COS/HEK wc	Yes					Cl^–		Smith et al., 2011
D112S	NpHv1 [D66S]	HEK wc	Yes					Cl^–		Chaves et al., 2016
D112S/R211S	hH_V1	Xenopus i-o	Yes			24	44	Gu⁺	At pH 8//8	Berger and Isacoff, 2011
D112F^a	hH_V1	COS/HEK wc	Yes	1.6	0.03	44^b	38	Cl^–		Musset et al., 2011
D112R/R211D	hH_V1	Xenopus i-o	Yes					H⁺		Berger and Isacoff, 2011
D112V	hH_V1	COS/HEK wc	No							Musset et al., 2011
D112L	hH_V1	Vesicle flux							Slows H⁺ flux	Letts, 2014
D112I^a	hH_V1	COS/HEK wc	No							DeCoursey, 2015b
D112Q	hH_V1	Xenopus i-o	No							Hong et al., 2014
D112A/L108D^a	hH_V1	COS/HEK wc	No							Morgan et al., 2013
D112V/V109D^a	hH_V1	COS/HEK wc	No							Morgan et al., 2013
D112A/V109D^a	hH_V1	COS/HEK wc	Yes					Cl^–		Morgan et al., 2013
D112A/V110D^a	hH_V1	COS/HEK wc	No							Morgan et al., 2013
D112A/L111D^a	hH_V1	COS/HEK wc	No							Morgan et al., 2013
D112A/A113D^a	hH_V1	COS/HEK wc	No							Morgan et al., 2013
D112A/L114D^a	hH_V1	COS/HEK wc	No							Morgan et al., 2013
D112A/L115D^a	hH_V1	COS/HEK wc	No							Morgan et al., 2013
D112A/V116D^a	hH_V1	COS/HEK wc	Yes					H⁺		Morgan et al., 2013
D112V/V116D^a	hH_V1	COS/HEK wc	Yes					H⁺		Morgan et al., 2013
D112V/V116E^a	hH_V1	COS/HEK wc	Yes					H⁺		Morgan et al., 2013
D112V/V116S^a	hH_V1	COS/HEK wc	Yes					Cl^–		Morgan et al., 2013
D112V/V116N^a	hH_V1	COS/HEK wc	Yes					Cl^–		Morgan et al., 2013
D112A/L117D^a	hH_V1	COS/HEK wc	No							Morgan et al., 2013
D112A/A118D^a	hH_V1	COS/HEK wc	No							Morgan et al., 2013
D112N/I127C	CiH_V1 [D160N/I175C]	Xenopus i-o	No							Mony et al., 2015
D112N/R211S/I127C	CiH_V1 [D160N/R261S/I175C]	Xenopus i-o	Yes			15				Mony et al., 2015
D112N/R211S/G199C	CiH_V1 [D160N/R261S/G249C]	Xenopus i-o	Yes			44				Mony et al., 2015
D112N/G199C	CiH_V1 [D160N/G249C]	Xenopus i-o	No							Mony et al., 2015
A113D^a	hH_V1	COS/HEK wc	Yes					H⁺		Morgan et al., 2013
E119A	hH_V1	HEK wc	Yes			20	47			Ramsey et al., 2010
E119L	hH_V1	Vesicle flux							nc H⁺ flux	Letts, 2014
E119S	mH_V1 [E115S]	HEK wc	Yes						nc Zn²⁺ inhibition	Takeshita et al., 2014
E119S/D123S	mH_V1 [E115S/D119S]	HEK wc	Yes						Weaker Zn²⁺ inhibition	Takeshita et al., 2014
E119A	CiH_V1 [E167A]	Xenopus TEVC	Yes			4				Chamberlin et al., 2014
E119C/R205C	CiH_V1 [E167C/R255C]	Xenopus TEVC	Yes			−2				Chamberlin et al., 2014
E119C/R208	CiH_V1 [E167C/R258C]	Xenopus TEVC	Yes			−52				Chamberlin et al., 2014
D123A	hH_V1	HEK wc	Yes			20	48			Ramsey et al., 2010
D123S	mH_V1 [D119S]	HEK wc	Yes						nc Zn²⁺ inhibition	Takeshita et al., 2014
D123C	CiH_V1 [D171C]	Xenopus i-o	Yes						MTS_o open> closed	Mony et al., 2015
D123A	CiH_V1 [D171A]	Xenopus TEVC	Yes			72				Chamberlin et al., 2014
D123A/R205N	CiH_V1 [D171A/R255N]	Xenopus TEVC	Yes			11				Chamberlin et al., 2014
K125A	hH_V1	HEK wc	Yes			19	47			Ramsey et al., 2010
K125C	CiH_V1 [K173C]	Xenopus i-o	Yes						MTS_o access	Mony et al., 2015
I127C	CiH_V1 [I175C]	Xenopus i-o	Yes			0				Mony et al., 2015
D130A	hH_V1	HEK wc	Yes			13				Ramsey et al., 2010
K131A	hH_V1	HEK wc	Yes			33				Ramsey et al., 2010

That numerical entries are shown does not imply that any given change was significant. Italicized mutant entries from nonhuman species show the hH_V1 equivalent. HEK, HEK-293, HEK-293T, tsA, or HM1; COS, COS-7; Xenopus, Xenopus laevis oocyte; wc, whole cell; i-o, inside-out patch configuration; TEVC, two-electrode voltage clamp. Blank entries indicate that the parameter was not examined. nc, measured, but no change. Parameters are given relative to WT in each study. For I?, yes means currents are detectable. Time constants are ratios of mutant/WT. The ΔV_threshold value is the change in absolute position of the g_H–V relationship versus WT. The ΔpH slope is the slope in millivolts of the relationship between V_threshold (or other parameters reflecting the absolute position of the g_H–V relationship) and V_rev or E_H (which are not identical; see section Table entries defined). For column Other, MTS access from inside or outside (MTS_i or MTS_o, respectively) is listed as open>closed if the open channel was more accessible.

In an H140A/H193A (Zn²⁺ insensitive) background.

Previously unpublished, analyzed from data for Musset et al. (2011).

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