Table 2.
Changes in HV1 properties in N-terminal (1–100) mutants versus WT channels
MutantSpeciesExpr. systemI?τactτtailΔVthrΔpH slopeSelectivityOtherReference
 hHV1S LK35.2 wc Yes 2.5 0.75 5.4 43.2 H+ More profound enhanced gating Hondares et al., 2014  
T9A hHV1S LK35.2 pp Yes 0.28 0.9 −18.2   Enhanced gating lost Hondares et al., 2014  
S77A hHV1S LK35.2 pp Yes 1.75 1.1 −3.6   nc Hondares et al., 2014  
T9A/S77A hHV1S LK35.2 pp Yes 0.63 0.8 −5.8   Enhanced gating lost Hondares et al., 2014  
T29A hHV1 LK35.2 pp Yes 0.23 1.8 −27.6   Enhanced gating lost Musset et al., 2010a  
T29D hHV1 LK35.2 pp Yes 0.41 1.0 −12.5   Enhanced gating lost Musset et al., 2010a  
M91T hHV1 COS wc Yes   20 47  First identified naturally occurring hHV1 mutation Iovannisci et al., 2010  
S97A hHV1 LK35.2 pp Yes 0.96 1.2 3.9   nc Musset et al., 2010a  
S97D hHV1 LK35.2 pp Yes 0.34 0.9 −3.0   nc Musset et al., 2010a  
T29A/S97A hHV1 LK35.2 pp Yes 0.25 1.7 −19.4   Enhanced gating lost Musset et al., 2010a  
S98A hHV1 HEK wc Yes   −7    Ramsey et al., 2010  
H99A hHV1 HEK wc Yes   13    Ramsey et al., 2010  
R100A hHV1 HEK wc Yes   −7    Ramsey et al., 2010  
ΔN (1–96 deleted) hHV1 HEK wc Yes   15 40   Ramsey et al., 2010  
ΔN/ΔC mHV1 (1–77 deleted, V216stop) HEK wc Yes 0.20  nc nc  Loss of dimer formation Koch et al., 2008  
ΔN/ΔC mHV1 (1–77 deleted, V216stop) HEK i-o Yes      Weaker Zn2+ effects Musset et al., 2010b  
MutantSpeciesExpr. systemI?τactτtailΔVthrΔpH slopeSelectivityOtherReference
 hHV1S LK35.2 wc Yes 2.5 0.75 5.4 43.2 H+ More profound enhanced gating Hondares et al., 2014  
T9A hHV1S LK35.2 pp Yes 0.28 0.9 −18.2   Enhanced gating lost Hondares et al., 2014  
S77A hHV1S LK35.2 pp Yes 1.75 1.1 −3.6   nc Hondares et al., 2014  
T9A/S77A hHV1S LK35.2 pp Yes 0.63 0.8 −5.8   Enhanced gating lost Hondares et al., 2014  
T29A hHV1 LK35.2 pp Yes 0.23 1.8 −27.6   Enhanced gating lost Musset et al., 2010a  
T29D hHV1 LK35.2 pp Yes 0.41 1.0 −12.5   Enhanced gating lost Musset et al., 2010a  
M91T hHV1 COS wc Yes   20 47  First identified naturally occurring hHV1 mutation Iovannisci et al., 2010  
S97A hHV1 LK35.2 pp Yes 0.96 1.2 3.9   nc Musset et al., 2010a  
S97D hHV1 LK35.2 pp Yes 0.34 0.9 −3.0   nc Musset et al., 2010a  
T29A/S97A hHV1 LK35.2 pp Yes 0.25 1.7 −19.4   Enhanced gating lost Musset et al., 2010a  
S98A hHV1 HEK wc Yes   −7    Ramsey et al., 2010  
H99A hHV1 HEK wc Yes   13    Ramsey et al., 2010  
R100A hHV1 HEK wc Yes   −7    Ramsey et al., 2010  
ΔN (1–96 deleted) hHV1 HEK wc Yes   15 40   Ramsey et al., 2010  
ΔN/ΔC mHV1 (1–77 deleted, V216stop) HEK wc Yes 0.20  nc nc  Loss of dimer formation Koch et al., 2008  
ΔN/ΔC mHV1 (1–77 deleted, V216stop) HEK i-o Yes      Weaker Zn2+ effects Musset et al., 2010b  

That numerical entries are shown does not imply that any given change was significant. The entries for hHV1S are in a short isoform and are compared with full-length hHV1. HEK, HEK-293, HEK-293T, tsA, or HM1; COS, COS-7; pp, perforated patch; wc, whole cell; i-o, inside-out patch configuration. Blank entries indicate that the parameter was not examined. nc, measured, but no change. Parameters are given relative to WT in each study. For I?, yes means currents are detectable. Time constants are ratios of mutant/WT. The ΔVthreshold value is the change in absolute position of the gH–V relationship versus WT. The ΔpH slope is the slope in millivolts of the relationship between Vthreshold (or other parameters reflecting the absolute position of the gH–V relationship) and Vrev or EH (which are not identical; see section Table entries defined). When C-terminal truncations (ΔC) are indicated as XNNNstop, this means STOP replaces X at position NNN; hence, position NNN and all subsequent residues are truncated, and the last position remaining is NNN-1. The mouse N-terminal deletions (ΔN) were done by replacing P78M to initiate translation at that position.

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