Table 1.
Buffer composition
Outward INa/CaInward INa/Ca
Loading bufferControl bufferActivation bufferLoading bufferControl bufferActivation buffer
30 mM HEPES 30 mM HEPES 30 mM HEPES  30 mM HEPES 30 mM HEPES 30 mM HEPES 
1 mM EGTA 1 mM EGTA 1 mM EGTA  1 mM EGTA 1 mM EGTA 1 mM EGTA 
4 mM MgCl2 4 mM MgCl2 4 mM MgCl2  4 mM MgCl2 4 mM MgCl2 4 mM MgCl2 
140 mM NaCl 280 mM sorbitol 280 mM sorbitol  220 mM sorbitol 180 mM sorbitol 180 mM sorbitol 
pH 7.4 (NMDG) pH 7.4 (NMDG) 100 µM CaCl2 (free)  20 mM CaCl2 60 mM choline Cl 50 mM choline Cl 
  pH 7.4 (NMDG)  pH 7.4 (NMDG) pH 7.4 (NMDG) 10 mM NaCl 
      pH 7.4 (NMDG) 
Outward INa/CaInward INa/Ca
Loading bufferControl bufferActivation bufferLoading bufferControl bufferActivation buffer
30 mM HEPES 30 mM HEPES 30 mM HEPES  30 mM HEPES 30 mM HEPES 30 mM HEPES 
1 mM EGTA 1 mM EGTA 1 mM EGTA  1 mM EGTA 1 mM EGTA 1 mM EGTA 
4 mM MgCl2 4 mM MgCl2 4 mM MgCl2  4 mM MgCl2 4 mM MgCl2 4 mM MgCl2 
140 mM NaCl 280 mM sorbitol 280 mM sorbitol  220 mM sorbitol 180 mM sorbitol 180 mM sorbitol 
pH 7.4 (NMDG) pH 7.4 (NMDG) 100 µM CaCl2 (free)  20 mM CaCl2 60 mM choline Cl 50 mM choline Cl 
  pH 7.4 (NMDG)  pH 7.4 (NMDG) pH 7.4 (NMDG) 10 mM NaCl 
      pH 7.4 (NMDG) 
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