Table 1. Buffer composition Outward I... | Rockefeller University Press

Table 1.

Buffer composition

Outward I_Na/Ca			Inward I_Na/Ca
Loading buffer	Control buffer	Activation buffer	Loading buffer	Control buffer	Activation buffer
30 mM HEPES	30 mM HEPES	30 mM HEPES	30 mM HEPES	30 mM HEPES	30 mM HEPES
1 mM EGTA	1 mM EGTA	1 mM EGTA	1 mM EGTA	1 mM EGTA	1 mM EGTA
4 mM MgCl₂	4 mM MgCl₂	4 mM MgCl₂	4 mM MgCl₂	4 mM MgCl₂	4 mM MgCl₂
140 mM NaCl	280 mM sorbitol	280 mM sorbitol	220 mM sorbitol	180 mM sorbitol	180 mM sorbitol
pH 7.4 (NMDG)	pH 7.4 (NMDG)	100 µM CaCl₂ (free)	20 mM CaCl₂	60 mM choline Cl	50 mM choline Cl
		pH 7.4 (NMDG)	pH 7.4 (NMDG)	pH 7.4 (NMDG)	10 mM NaCl
					pH 7.4 (NMDG)

Outward I_Na/Ca			Inward I_Na/Ca
Loading buffer	Control buffer	Activation buffer	Loading buffer	Control buffer	Activation buffer
30 mM HEPES	30 mM HEPES	30 mM HEPES	30 mM HEPES	30 mM HEPES	30 mM HEPES
1 mM EGTA	1 mM EGTA	1 mM EGTA	1 mM EGTA	1 mM EGTA	1 mM EGTA
4 mM MgCl₂	4 mM MgCl₂	4 mM MgCl₂	4 mM MgCl₂	4 mM MgCl₂	4 mM MgCl₂
140 mM NaCl	280 mM sorbitol	280 mM sorbitol	220 mM sorbitol	180 mM sorbitol	180 mM sorbitol
pH 7.4 (NMDG)	pH 7.4 (NMDG)	100 µM CaCl₂ (free)	20 mM CaCl₂	60 mM choline Cl	50 mM choline Cl
		pH 7.4 (NMDG)	pH 7.4 (NMDG)	pH 7.4 (NMDG)	10 mM NaCl
					pH 7.4 (NMDG)