Table 2. Time constants of current... | Rockefeller University Press

Table 2.

Time constants of current activation

Constructs	Kinetics	Time constant (ms)			P	N
		Mean	SD	SEM
Ca_V1.1a	slow	43.3	25.8	4.7	na,^†††	30
	fast	7.4	3.6	0.6	na,ns
Ca_V1.1e	slow	22.6	13.9	3.1	***,na	20
	fast	4.4	2.3	0.5	ns,na
Ca_V1.1a-L_1212/1215N	slow	16.5	7.4	2.6	***,ns	8
	fast	4.5	2.6	0.9	ns,ns
Ca_V1.1e-L_I		4.1	3.0	0.6	na	25
Ca_V1.1e-L4_I		2.7	1.3	0.5	ns	6
Ca_V1.1e-3L_I		4.1	1.6	0.6	ns	7
Ca_V1.1a-CS_I		3.3	0.9	0.3	ns	12
Ca_V1.1e-CS_I	mono	4.7	2.1	0.8	ns	7
Ca_V1.1e-L_I-3L_IV		4.0	1.9	0.5	ns	14
Ca_V1.1e-L_I-L4_IV		10.4	2.7	0.8	°°°	12
Ca_V1.1e-L_I-L_IV		4.1	1.6	0.4	ns	18
Ca_V1.1e-L_I-3L4_IV		2.6	0.7	0.2	ns	7

Constructs	Kinetics	Time constant (ms)			P	N
		Mean	SD	SEM
Ca_V1.1a	slow	43.3	25.8	4.7	na,^†††	30
	fast	7.4	3.6	0.6	na,ns
Ca_V1.1e	slow	22.6	13.9	3.1	***,na	20
	fast	4.4	2.3	0.5	ns,na
Ca_V1.1a-L_1212/1215N	slow	16.5	7.4	2.6	***,ns	8
	fast	4.5	2.6	0.9	ns,ns
Ca_V1.1e-L_I		4.1	3.0	0.6	na	25
Ca_V1.1e-L4_I		2.7	1.3	0.5	ns	6
Ca_V1.1e-3L_I		4.1	1.6	0.6	ns	7
Ca_V1.1a-CS_I		3.3	0.9	0.3	ns	12
Ca_V1.1e-CS_I	mono	4.7	2.1	0.8	ns	7
Ca_V1.1e-L_I-3L_IV		4.0	1.9	0.5	ns	14
Ca_V1.1e-L_I-L4_IV		10.4	2.7	0.8	°°°	12
Ca_V1.1e-L_I-L_IV		4.1	1.6	0.4	ns	18
Ca_V1.1e-L_I-3L4_IV		2.6	0.7	0.2	ns	7

The activation of Ca_V1.1a, Ca_V1.1e, and Ca_V1.1a-L_1212/1215N was fitted by a double-exponential function, whereas all other currents were best fitted with a mono-exponential function. Numerical values represent mean, SD, and SEM. Statistical differences between mutants, chimeras, and Ca_V1.1a or Ca_V1.1e were measured with one-way ANOVA, followed by a Bonferroni multiple comparison test. Significant differences against Ca_V1.1a are indicated as ***, P < 0.0001. Differences against Ca_V1.1e are indicated as ^†††, P < 0.0001. For mono-exponential time constants, differences against Ca_V1.1e-L_I are indicated as °°°, P < 0.0001. ns, not significant (P < 0.05); na, not applicable.

[ViewLarge]

Sharing Unavailable