Table 3. Relative anion permeabilities... | Rockefeller University Press

Table 3.

Relative anion permeabilities of WT EAAT1 and R477H

pH	Transporter	E_rev				P_X⁻/P_Cl⁻
pH	Transporter	Cl⁻	Br⁻	I⁻	NO₃⁻	P_Br⁻/P_Cl⁻	P_I⁻/P_Cl⁻	P_NO3⁻/P_Cl⁻
		mV	mV	mV	mV
pH 5.5	EAAT1	51 ± 4	28 ± 3	−4 ± 3	−10 ± 3	2.5 ± 0.1	9.0 ± 0.5	11.1 ± 0.6
	R477H	14 ± 2	0 ± 1	−29 ± 1	−25 ± 2	1.75 ± 0.07	5.8 ± 0.4	5.1 ± 0.5
pH 8.5	EAAT1	50 ± 2	46 ± 2	17 ± 2	13 ± 2	1.19 ± 0.03	3.8 ± 0.2	4.4 ± 0.3
	R477H	−21 ± 3	−21 ± 3	−24 ± 3	−24 ± 2	ND	ND	ND

pH	Transporter	E_rev				P_X⁻/P_Cl⁻
pH	Transporter	Cl⁻	Br⁻	I⁻	NO₃⁻	P_Br⁻/P_Cl⁻	P_I⁻/P_Cl⁻	P_NO3⁻/P_Cl⁻
		mV	mV	mV	mV
pH 5.5	EAAT1	51 ± 4	28 ± 3	−4 ± 3	−10 ± 3	2.5 ± 0.1	9.0 ± 0.5	11.1 ± 0.6
	R477H	14 ± 2	0 ± 1	−29 ± 1	−25 ± 2	1.75 ± 0.07	5.8 ± 0.4	5.1 ± 0.5
pH 8.5	EAAT1	50 ± 2	46 ± 2	17 ± 2	13 ± 2	1.19 ± 0.03	3.8 ± 0.2	4.4 ± 0.3
	R477H	−21 ± 3	−21 ± 3	−24 ± 3	−24 ± 2	ND	ND	ND

Reversal potentials (E_rev) of saturating l-glutamate–elicited currents were obtained in buffers containing 10 mM Cl⁻, Br⁻, I⁻, and NO₃⁻ salts. Relative anion permeabilities were calculated from E_rev measurements using the Goldman–Hodgkin–Katz equation (Eq. 3). ND indicates that no relative anion permeability could be measured as Cl⁻ is not permeant through R477H at pH 8.5. Data shown represent the mean ± SEM (n ≥ 4).

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