Table 1. Substrate transport properties... | Rockefeller University Press

Table 1.

Substrate transport properties of WT EAAT1 and R477H

Substrate	Transporter	K_0.5
Substrate	Transporter	pH 5.5	pH 6.5	pH 7.5	pH 8.5
		µM	µM	µM	µM
l-glutamate	WT EAAT1	32 ± 3	25 ± 3	28 ± 6	41 ± 2
	R477H	0.70 ± 0.03	1.6 ± 0.2	9 ± 2	64 ± 8
l-aspartate	WT EAAT1	14 ± 2	19 ± 2	15 ± 3	33 ± 3
	R477H	2.0 ± 0.4	6.2 ± 0.4	13 ± 3	61 ± 3

Substrate	Transporter	K_0.5
Substrate	Transporter	pH 5.5	pH 6.5	pH 7.5	pH 8.5
		µM	µM	µM	µM
l-glutamate	WT EAAT1	32 ± 3	25 ± 3	28 ± 6	41 ± 2
	R477H	0.70 ± 0.03	1.6 ± 0.2	9 ± 2	64 ± 8
l-aspartate	WT EAAT1	14 ± 2	19 ± 2	15 ± 3	33 ± 3
	R477H	2.0 ± 0.4	6.2 ± 0.4	13 ± 3	61 ± 3

l-Glutamate and l-aspartate concentration responses were measured in ND96 at pH 5.5, 6.5, 7.5, and 8.5 and fitted by least squares as a function of current at −60 mV to the Michaelis–Menten equation (Eq. 1) to obtain K_0.5 values for both glutamate and aspartate. Data shown represent the mean ± SEM (n ≥ 4).

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