| Component . | . | Solution name . | |||||||
|---|---|---|---|---|---|---|---|---|---|
| L . | M . | N . | O . | P . | Q . | R . | S . | ||
| mM | mM | mM | mM | mM | mM | mM | mM | ||
| Na+ | 100 | 140 | 140 | ||||||
| NMDG+ | 100 | 100 | 140 | 140 | 125 | ||||
| Ca2+ | 1 | 1 | 1 | 1 | |||||
| Mg2+ | 1 | 1 | 1 | 2 | 2 | 2 | 1 | 1 | |
| Choline | 25 | 25 | 25 | 15 | |||||
| Cl− | 42 | 4 | 4 | 15 | 4 | 4 | 4 | 4 | |
| HCO3− | 25 | 25 | 25 | 15 | 25 | ||||
| Glutamate | 60 | 100 | 100 | 129 | 140 | 125 | 140 | 115 | |
| EGTA | 10 | 1 | 1 | 1 | |||||
| Tris | 10 | 1 | 1 | 1 | |||||
| Glucose | 5 | 5 | 5 | 5 | |||||
| HEPES | 10 | 10 | 10 | 1 | 1 | 1 | 10 | 10 | |
| Component . | . | Solution name . | |||||||
|---|---|---|---|---|---|---|---|---|---|
| L . | M . | N . | O . | P . | Q . | R . | S . | ||
| mM | mM | mM | mM | mM | mM | mM | mM | ||
| Na+ | 100 | 140 | 140 | ||||||
| NMDG+ | 100 | 100 | 140 | 140 | 125 | ||||
| Ca2+ | 1 | 1 | 1 | 1 | |||||
| Mg2+ | 1 | 1 | 1 | 2 | 2 | 2 | 1 | 1 | |
| Choline | 25 | 25 | 25 | 15 | |||||
| Cl− | 42 | 4 | 4 | 15 | 4 | 4 | 4 | 4 | |
| HCO3− | 25 | 25 | 25 | 15 | 25 | ||||
| Glutamate | 60 | 100 | 100 | 129 | 140 | 125 | 140 | 115 | |
| EGTA | 10 | 1 | 1 | 1 | |||||
| Tris | 10 | 1 | 1 | 1 | |||||
| Glucose | 5 | 5 | 5 | 5 | |||||
| HEPES | 10 | 10 | 10 | 1 | 1 | 1 | 10 | 10 | |
The pH value for solutions L–N, R, and S was 7.4. The pH value for solutions O–Q was 7.2.