Table 2. Model differential equations... | Rockefeller University Press

Table 2.

Model differential equations

Reaction	Flux
PI to PI(4)P	k_4K * [PI] − k_4P * [PIP]
PI(4)P to PI(4,5)P₂	k_5K * [PIP] − k_5P * [PIP₂]
PLC (basal)	[PIP₂] * (PLC_basal + k_ _PLC * fold PIP₂ * Ga_GTP_PLC), where PLC_basal = 0.0001 s⁻¹, k__PLC = 0.2 µm²molecule⁻¹s⁻¹ and fold PIP₂ = 3
PLC (stimulated)	[PIP₂] * (PLC_basal + k__PLC * fold PIP₂ * Ga_GTP_PLC) + [PIP₂] * PLC_stim * Ga_GTP_PLC * [Ca_C/(Ca_C + K_d_PLC_Ca)], where PLC_basal = 0.0001 s⁻¹, k__PLC = 0.2 µm²molecule⁻¹s⁻¹, fold PIP₂ = 3, PLC_stim = 7 s⁻¹, and K_d_PLC_Ca = 1 µM
RL to RLP	k_{f_RL} * [RL] * (weighting_factor_PKC_DAG * [PKC_DAG] + weighting_factor_GRK * [GRK]) − k_{r_RLP} * [RLP]
PKC to active PKC	k_{f_PKC_DAG} * [PKC] * [DAG] − k_{r_PKC_DAG} * [PKC_DAG]
R to RP	k_{f_R} * [R] * [PKC_DAG] − k_{r_RP} * [RP]
RP to RLP	k_{f_L2} * [RP] − k_{r_L2} * [RLP]
RLP to RLPA1	k_{f_RLP} * [RLP] * [Arrestin1] − k_{r_RLPA1} * [RLPA1]
RLP to RLPA2	k_{f_RLP} * [RLP] * [Arrestin2] − k_{r_RLPA2} * [RLPA2]

Reaction	Flux
PI to PI(4)P	k_4K * [PI] − k_4P * [PIP]
PI(4)P to PI(4,5)P₂	k_5K * [PIP] − k_5P * [PIP₂]
PLC (basal)	[PIP₂] * (PLC_basal + k_ _PLC * fold PIP₂ * Ga_GTP_PLC), where PLC_basal = 0.0001 s⁻¹, k__PLC = 0.2 µm²molecule⁻¹s⁻¹ and fold PIP₂ = 3
PLC (stimulated)	[PIP₂] * (PLC_basal + k__PLC * fold PIP₂ * Ga_GTP_PLC) + [PIP₂] * PLC_stim * Ga_GTP_PLC * [Ca_C/(Ca_C + K_d_PLC_Ca)], where PLC_basal = 0.0001 s⁻¹, k__PLC = 0.2 µm²molecule⁻¹s⁻¹, fold PIP₂ = 3, PLC_stim = 7 s⁻¹, and K_d_PLC_Ca = 1 µM
RL to RLP	k_{f_RL} * [RL] * (weighting_factor_PKC_DAG * [PKC_DAG] + weighting_factor_GRK * [GRK]) − k_{r_RLP} * [RLP]
PKC to active PKC	k_{f_PKC_DAG} * [PKC] * [DAG] − k_{r_PKC_DAG} * [PKC_DAG]
R to RP	k_{f_R} * [R] * [PKC_DAG] − k_{r_RP} * [RP]
RP to RLP	k_{f_L2} * [RP] − k_{r_L2} * [RLP]
RLP to RLPA1	k_{f_RLP} * [RLP] * [Arrestin1] − k_{r_RLPA1} * [RLPA1]
RLP to RLPA2	k_{f_RLP} * [RLP] * [Arrestin2] − k_{r_RLPA2} * [RLPA2]

Many additional reactions that have been already described previously in our paper are not in this table. The parameters are in previous papers (Dickson et al., 2013; Falkenburger et al., 2013). We added Ca²⁺-sensitive PLC activation in our model. In this table and calculation, the units of concentration and area are µM and µm², respectively.

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