Elk1 Boltzmann fit parameters for standard G-V curves measured from a −100-mV hold
| Channel | Parameter | On cell | Excised | 10 µM PIP2 | ||||||
| Mean | SEM | na | Mean | SEM | n | Mean | SEM | n | ||
| WT | V50b | −33.9 | 1.1 | 32 | −52.5 | 0.8 | 37 | −30.8 | 0.8 | 37 |
| sc | 12.1 | 0.2 | 32 | 8.7 | 0.1 | 37 | 10.2 | 0.1 | 37 | |
| Δ2-136 | V50 | −8.1 | 1.8 | 15 | −45.2 | 1.0 | 15 | −13.7 | 0.8 | 14 |
| s | 10.1 | 0.3 | 15 | 8.0 | 0.3 | 15 | 8.4 | 0.3 | 14 | |
| K5Q | V50 | −22.6 | 1.2 | 9 | −55.9 | 1.3 | 9 | −26.1 | 1.3 | 8 |
| s | 9.7 | 0.2 | 9 | 8.2 | 0.0 | 9 | 8.7 | 0.2 | 8 | |
| R347E | V50 | −45.2 | 2.3 | 18 | −46.4 | 2.2 | 18 | −25.8 | 2.9 | 15 |
| s | 16.4 | 0.3 | 18 | 14.1 | 0.2 | 18 | 16.2 | 0.4 | 15 | |
| R347C | V50 | −20.6 | 2.4 | 17 | −26.4 | 1.7 | 17 | −3.5 | 1.8 | 12 |
| s | 12.1 | 0.3 | 17 | 13.3 | 0.3 | 17 | 14.3 | 0.4 | 12 | |
| R475E | V50 | −38.5 | 1.6 | 17 | −37.5 | 1.0 | 20 | −25.1 | 1.4 | 17 |
| s | 12.9 | 0.4 | 17 | 11.4 | 0.2 | 20 | 12.2 | 0.2 | 17 | |
| R479E | V50 | −59.6 | 2.3 | 17 | −38.3 | 1.3 | 20 | −43.0 | 2.0 | 14 |
| s | 14.4 | 0.5 | 17 | 13.1 | 0.2 | 20 | 15.9 | 0.4 | 14 | |
| R479Q | V50 | −53.7 | 2.8 | 11 | −47.6 | 1.8 | 12 | −42.5 | 2.5 | 11 |
| s | 14.9 | 0.3 | 11 | 10.4 | 0.2 | 12 | 14.2 | 0.4 | 11 | |
| Channel | Parameter | On cell | Excised | 10 µM PIP2 | ||||||
| Mean | SEM | na | Mean | SEM | n | Mean | SEM | n | ||
| WT | V50b | −33.9 | 1.1 | 32 | −52.5 | 0.8 | 37 | −30.8 | 0.8 | 37 |
| sc | 12.1 | 0.2 | 32 | 8.7 | 0.1 | 37 | 10.2 | 0.1 | 37 | |
| Δ2-136 | V50 | −8.1 | 1.8 | 15 | −45.2 | 1.0 | 15 | −13.7 | 0.8 | 14 |
| s | 10.1 | 0.3 | 15 | 8.0 | 0.3 | 15 | 8.4 | 0.3 | 14 | |
| K5Q | V50 | −22.6 | 1.2 | 9 | −55.9 | 1.3 | 9 | −26.1 | 1.3 | 8 |
| s | 9.7 | 0.2 | 9 | 8.2 | 0.0 | 9 | 8.7 | 0.2 | 8 | |
| R347E | V50 | −45.2 | 2.3 | 18 | −46.4 | 2.2 | 18 | −25.8 | 2.9 | 15 |
| s | 16.4 | 0.3 | 18 | 14.1 | 0.2 | 18 | 16.2 | 0.4 | 15 | |
| R347C | V50 | −20.6 | 2.4 | 17 | −26.4 | 1.7 | 17 | −3.5 | 1.8 | 12 |
| s | 12.1 | 0.3 | 17 | 13.3 | 0.3 | 17 | 14.3 | 0.4 | 12 | |
| R475E | V50 | −38.5 | 1.6 | 17 | −37.5 | 1.0 | 20 | −25.1 | 1.4 | 17 |
| s | 12.9 | 0.4 | 17 | 11.4 | 0.2 | 20 | 12.2 | 0.2 | 17 | |
| R479E | V50 | −59.6 | 2.3 | 17 | −38.3 | 1.3 | 20 | −43.0 | 2.0 | 14 |
| s | 14.4 | 0.5 | 17 | 13.1 | 0.2 | 20 | 15.9 | 0.4 | 14 | |
| R479Q | V50 | −53.7 | 2.8 | 11 | −47.6 | 1.8 | 12 | −42.5 | 2.5 | 11 |
| s | 14.9 | 0.3 | 11 | 10.4 | 0.2 | 12 | 14.2 | 0.4 | 11 | |