Table 1. Anion selectivity sequence of... | Rockefeller University Press

Table 1.

Anion selectivity sequence of CLC-2-based on reversal potentials

Anion	Extracellular			Intracellular
	ΔVr	P_x/P_Cl	n	Vr	P_x/P_Cl	n
	mV			mV
Cl⁻	0	1	28	−5.4 ± 0.9	1	28
SCN⁻	−11.4 ± 3.6	1.62 ± 0.22	5	15 ± 0.84	1.84 ± 0.06	6
Br⁻	5.0 ± 0.71	0.82 ± 0.02	5	0.5 ± 2.0	1.05 ± 0.08	12
I⁻	37.6 ± 1.7	0.23 ± 0.01	5	−20.5 ± 2.4	0.46 ± 0.04	4
F⁻	36.00 ± 0.63	0.00 ± 0.01	5	−34.65 ± 1.97	0.01 ± 0.02	5
Glu⁻	34.53 ± 1.19	0.01 ± 0.01	5	−32.80 ± 0.17	0.03 ± 0.00	4
Methanesulfonate	31.17 ± 1.11	0.06 ± 0.01	5	−31.10 ± 1.16	0.06 ± 0.02	6
Gluconate	30.55 ± 0.36	0.06 ± 0.01	4	−32.05 ± 1.42	0.05 ± 0.02	6
Ace⁻	33.11 ± 0.92	0.03 ± 0.01	4	−32.40 ± 0.77	0.04 ± 0.01	6
SO₄²⁻	35.89 ± 0.59	0.00 ± 0.00	3	−36.06 ± 1.02	0.00 ± 0.01	6
	SCN⁻ > Cl⁻ > Br⁻ > I⁻ > methanesulfonate = gluconate ≥ Ace⁻ = Glu⁻ = F⁻ = SO₄²⁻			SCN⁻ > Br⁻ = Cl⁻ > I⁻ > methanesulfonate = gluconate = Ace⁻ = Glu⁻ = F⁻ ≥ SO₄²⁻

Anion	Extracellular			Intracellular
	ΔVr	P_x/P_Cl	n	Vr	P_x/P_Cl	n
	mV			mV
Cl⁻	0	1	28	−5.4 ± 0.9	1	28
SCN⁻	−11.4 ± 3.6	1.62 ± 0.22	5	15 ± 0.84	1.84 ± 0.06	6
Br⁻	5.0 ± 0.71	0.82 ± 0.02	5	0.5 ± 2.0	1.05 ± 0.08	12
I⁻	37.6 ± 1.7	0.23 ± 0.01	5	−20.5 ± 2.4	0.46 ± 0.04	4
F⁻	36.00 ± 0.63	0.00 ± 0.01	5	−34.65 ± 1.97	0.01 ± 0.02	5
Glu⁻	34.53 ± 1.19	0.01 ± 0.01	5	−32.80 ± 0.17	0.03 ± 0.00	4
Methanesulfonate	31.17 ± 1.11	0.06 ± 0.01	5	−31.10 ± 1.16	0.06 ± 0.02	6
Gluconate	30.55 ± 0.36	0.06 ± 0.01	4	−32.05 ± 1.42	0.05 ± 0.02	6
Ace⁻	33.11 ± 0.92	0.03 ± 0.01	4	−32.40 ± 0.77	0.04 ± 0.01	6
SO₄²⁻	35.89 ± 0.59	0.00 ± 0.00	3	−36.06 ± 1.02	0.00 ± 0.01	6
	SCN⁻ > Cl⁻ > Br⁻ > I⁻ > methanesulfonate = gluconate ≥ Ace⁻ = Glu⁻ = F⁻ = SO₄²⁻			SCN⁻ > Br⁻ = Cl⁻ > I⁻ > methanesulfonate = gluconate = Ace⁻ = Glu⁻ = F⁻ ≥ SO₄²⁻

Reversal potentials in the presence of poorly permeant anions were determined in solutions containing 25% Cl⁻ plus 75% of targeted poorly permeant anion.