Charge movement and Ca2+ release fit parameters
| Construct | Q-V | ΔF/F-V | ||||
| Qmax | VQ | kQ | ΔF/Fmax | VF | kF | |
| nC/µF | mV | mV | ΔF/F | mV | mV | |
| YFP | 22.8 ± 3.4 (5) | −7.8 ± 3.5 | 12.0 ± 1.7 | 1.6 ± 0.2 (6) | −13.0 ± 1.2 | 8.4 ± 0.8 |
| V-Rem | 22.4 ± 1.2 (7) | −8.1 ± 1.6 | 11.8 ± 1.6 | 0.6 ± 0.1a (8) | −20.2 ± 4.7 | 14.7 ± 2.6 |
| V-Rem AAA | 24.6 ± 3.4 (5) | −8.5 ± 1.0 | 8.1 ± 1.0 | 1.4 ± 0.2 (6) | −15.5 ± 1.3 | 9.9 ± 0.9 |
| Construct | Q-V | ΔF/F-V | ||||
| Qmax | VQ | kQ | ΔF/Fmax | VF | kF | |
| nC/µF | mV | mV | ΔF/F | mV | mV | |
| YFP | 22.8 ± 3.4 (5) | −7.8 ± 3.5 | 12.0 ± 1.7 | 1.6 ± 0.2 (6) | −13.0 ± 1.2 | 8.4 ± 0.8 |
| V-Rem | 22.4 ± 1.2 (7) | −8.1 ± 1.6 | 11.8 ± 1.6 | 0.6 ± 0.1a (8) | −20.2 ± 4.7 | 14.7 ± 2.6 |
| V-Rem AAA | 24.6 ± 3.4 (5) | −8.5 ± 1.0 | 8.1 ± 1.0 | 1.4 ± 0.2 (6) | −15.5 ± 1.3 | 9.9 ± 0.9 |
Data are given as mean ± SEM, with the numbers in parentheses indicating the number of FDB fibers tested. Charge movement and EC coupling data were fit by Eqs. 1 and 3, respectively. Only cells with Ca2+ transients that could be fit with Eq. 3 were used for analysis; two Rem-expressing fibers lacking quantifiable ΔF/F were dropped. One significant difference between the three groups is indicated.
P < 0.001; one-way ANOVA.