Table 1.

Composition of solutions used in this study

No. Sodium chloride Magnesium chloride Potassium chloride Sodium glutamate monohydrate HEPES or MES EGTA 
I 130 0 0 0 3 0.2 
II 30 100 0 0 3 0.2 
III 0 0 130 0 3 0.2 
IV 0 0 0 130 3 0.2 
No. Sodium chloride Magnesium chloride Potassium chloride Sodium glutamate monohydrate HEPES or MES EGTA 
I 130 0 0 0 3 0.2 
II 30 100 0 0 3 0.2 
III 0 0 130 0 3 0.2 
IV 0 0 0 130 3 0.2 

All values indicate the final concentration (mM) after adjustment of the pH level. For Solutions I, II, and III, pH was adjusted by hydrochloric acid or sodium hydroxide, whereas for Solution IV, pH was adjusted by l-glutamic acid or sodium hydroxide.

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