LRET lifetimes and measurements
| Ligated State (n) | Donor-only lifetime | Donor-acceptor lifetime | Distance |
| µs | µs | Å | |
| GluN2B Cleft – GluN1* + GluN2Bcleft (Ni(NTA)2Cy3) (51.0 Å) | |||
| Apo (3) | 1,847 ± 1 | 252 ± 12 | 48.8 ± 0.4 |
| Ifenprodil (4) | 1,826 ± 1 | 203 ± 12 | 46.0 ± 0.5 |
| GluN1 Cleft – GluN1cleft + GluN2B* (Alexa Fluor 555) (47.2 Å) | |||
| Apo (2) | 1,740 ± 1 | 275 ± 4 | 49.2 ± 0.1 |
| Ifenprodil (2) | 1,747 ± 1 | 263 ± 2 | 48.7 ± 0.1 |
| GluN1–GluN2B Upper Lobes – GluN1*C22 + GluN2B*H30 (Ni(NTA)2Cy3) (64.0 Å) | |||
| Apo (5) | 1,738 ± 1 | 362 ± 26 | 52.1 ± 0.6 |
| Ifenprodil (2) | 1,807 ± 1 | 374 ± 26 | 52.0 ± 0.6 |
| GluN1 Upper Lobe–GluN2B Lower Lobe – GluN1*C22 + GluN2B*232 (Alexa Fluor 555) (35.2 Å) | |||
| Apo (3) | 1,674 ± 16 | 228 ± 9 | 47.8 ± 0.3 |
| Ifenprodil (3) | 1,694 ± 4 | 194 ± 8 | 46.2 ± 0.3 |
| GluN1 Upper Lobe–GluN2A Lower Lobe – GluN1*C22 + GluN2A*C231 (Alexa Fluor 555) | |||
| Apo (3) | 1,692 ± 1 | 187 ± 6 | 45.9 ± 0.2 |
| Zinc (2) | 1,683 ± 1 | 156 ± 9 | 44.4 ± 0.4 |
| Ligated State (n) | Donor-only lifetime | Donor-acceptor lifetime | Distance |
| µs | µs | Å | |
| GluN2B Cleft – GluN1* + GluN2Bcleft (Ni(NTA)2Cy3) (51.0 Å) | |||
| Apo (3) | 1,847 ± 1 | 252 ± 12 | 48.8 ± 0.4 |
| Ifenprodil (4) | 1,826 ± 1 | 203 ± 12 | 46.0 ± 0.5 |
| GluN1 Cleft – GluN1cleft + GluN2B* (Alexa Fluor 555) (47.2 Å) | |||
| Apo (2) | 1,740 ± 1 | 275 ± 4 | 49.2 ± 0.1 |
| Ifenprodil (2) | 1,747 ± 1 | 263 ± 2 | 48.7 ± 0.1 |
| GluN1–GluN2B Upper Lobes – GluN1*C22 + GluN2B*H30 (Ni(NTA)2Cy3) (64.0 Å) | |||
| Apo (5) | 1,738 ± 1 | 362 ± 26 | 52.1 ± 0.6 |
| Ifenprodil (2) | 1,807 ± 1 | 374 ± 26 | 52.0 ± 0.6 |
| GluN1 Upper Lobe–GluN2B Lower Lobe – GluN1*C22 + GluN2B*232 (Alexa Fluor 555) (35.2 Å) | |||
| Apo (3) | 1,674 ± 16 | 228 ± 9 | 47.8 ± 0.3 |
| Ifenprodil (3) | 1,694 ± 4 | 194 ± 8 | 46.2 ± 0.3 |
| GluN1 Upper Lobe–GluN2A Lower Lobe – GluN1*C22 + GluN2A*C231 (Alexa Fluor 555) | |||
| Apo (3) | 1,692 ± 1 | 187 ± 6 | 45.9 ± 0.2 |
| Zinc (2) | 1,683 ± 1 | 156 ± 9 | 44.4 ± 0.4 |
Shown are the lifetimes and distances from measurements in GluN1/GluN2B receptors in the apo, ifenprodil-bound, and zinc-bound states. The distance next to the construct name is measured between the alpha carbons of the residues labeled from the ifenprodil-bound structure of the NMDA receptor (Protein Data Bank accession no. 4PE5).