Mutations made and acceptor fluorophores
| Construct name | Mutations | Construct coexpressed with | Acceptor fluorophore |
| GluN1* | C22S, C459S | GluN2Bcleft | Ni(NTA)2Cy3 |
| GluN1*C22 | C459S | GluN2B*H30 | Ni(NTA)2Cy3 |
| GluN2B*232 | Alexa Fluor 555 | ||
| GluN2A*231 | Alexa Fluor 555 | ||
| GluN1cleft | S224C, C459S | GluN2B* | Alexa Fluor 555 |
| GluN2A*231 | C395S, C461S | GluN1*C22 | Alexa Fluor 555 |
| GluN2B* | C232S, C399S, C495S | GluN1cleft | Alexa Fluor 555 |
| GluN2B*H30 | C232S, C399S, C495S, insertion of hexa-his tag followed by amino acids LVPRGS after residue 30 | GluN1*C22 | Ni(NTA)2Cy3 |
| GluN2Bcleft | C399S, C495S, insertion of hexa-his tag followed by amino acids LVPRGS after residue 30 | GluN1* | Ni(NTA)2Cy3 |
| GluN2B*231 | C399S, C495S | GluN1*C22 | Alexa Fluor 555 |
| Construct name | Mutations | Construct coexpressed with | Acceptor fluorophore |
| GluN1* | C22S, C459S | GluN2Bcleft | Ni(NTA)2Cy3 |
| GluN1*C22 | C459S | GluN2B*H30 | Ni(NTA)2Cy3 |
| GluN2B*232 | Alexa Fluor 555 | ||
| GluN2A*231 | Alexa Fluor 555 | ||
| GluN1cleft | S224C, C459S | GluN2B* | Alexa Fluor 555 |
| GluN2A*231 | C395S, C461S | GluN1*C22 | Alexa Fluor 555 |
| GluN2B* | C232S, C399S, C495S | GluN1cleft | Alexa Fluor 555 |
| GluN2B*H30 | C232S, C399S, C495S, insertion of hexa-his tag followed by amino acids LVPRGS after residue 30 | GluN1*C22 | Ni(NTA)2Cy3 |
| GluN2Bcleft | C399S, C495S, insertion of hexa-his tag followed by amino acids LVPRGS after residue 30 | GluN1* | Ni(NTA)2Cy3 |
| GluN2B*231 | C399S, C495S | GluN1*C22 | Alexa Fluor 555 |
This table describes all the mutations present in each construct, the construct it was coexpressed with, and the acceptor fluorophore used in the LRET experiments.