Table 1. Mutations made and acceptor... | Rockefeller University Press

Table 1.

Mutations made and acceptor fluorophores

Construct name	Mutations	Construct coexpressed with	Acceptor fluorophore
GluN1*	C22S, C459S	GluN2B^cleft	Ni(NTA)₂Cy3
GluN1*^C22	C459S	GluN2B*^H30	Ni(NTA)₂Cy3
		GluN2B*²³²	Alexa Fluor 555
		GluN2A*²³¹	Alexa Fluor 555
GluN1^cleft	S224C, C459S	GluN2B*	Alexa Fluor 555
GluN2A*²³¹	C395S, C461S	GluN1*^C22	Alexa Fluor 555
GluN2B*	C232S, C399S, C495S	GluN1^cleft	Alexa Fluor 555
GluN2B*^H30	C232S, C399S, C495S, insertion of hexa-his tag followed by amino acids LVPRGS after residue 30	GluN1*^C22	Ni(NTA)₂Cy3
GluN2B^cleft	C399S, C495S, insertion of hexa-his tag followed by amino acids LVPRGS after residue 30	GluN1*	Ni(NTA)₂Cy3
GluN2B*²³¹	C399S, C495S	GluN1*^C22	Alexa Fluor 555

Construct name	Mutations	Construct coexpressed with	Acceptor fluorophore
GluN1*	C22S, C459S	GluN2B^cleft	Ni(NTA)₂Cy3
GluN1*^C22	C459S	GluN2B*^H30	Ni(NTA)₂Cy3
		GluN2B*²³²	Alexa Fluor 555
		GluN2A*²³¹	Alexa Fluor 555
GluN1^cleft	S224C, C459S	GluN2B*	Alexa Fluor 555
GluN2A*²³¹	C395S, C461S	GluN1*^C22	Alexa Fluor 555
GluN2B*	C232S, C399S, C495S	GluN1^cleft	Alexa Fluor 555
GluN2B*^H30	C232S, C399S, C495S, insertion of hexa-his tag followed by amino acids LVPRGS after residue 30	GluN1*^C22	Ni(NTA)₂Cy3
GluN2B^cleft	C399S, C495S, insertion of hexa-his tag followed by amino acids LVPRGS after residue 30	GluN1*	Ni(NTA)₂Cy3
GluN2B*²³¹	C399S, C495S	GluN1*^C22	Alexa Fluor 555

This table describes all the mutations present in each construct, the construct it was coexpressed with, and the acceptor fluorophore used in the LRET experiments.