Table 2.

Negative fixed charge density (molarity) of channels

Channel 100 mM KCl 250 mM KCl Mean 
WT (PA63)7 0.70 0.85 0.78 
(PA63E302S)7 0.60 0.83 0.72 
(PA63E343S)7 0.68 0.80 0.74 
(PA63D276S)7 0.70 0.74 0.72 
(PA63D335S)7 0.58 0.77 0.68 
(PA63E308S)7 0.55 0.72 0.64 
(PA63D315S)7 0.50 0.63 0.57 
(PA63F427A)7 0.38 0.48 0.43 
Channel 100 mM KCl 250 mM KCl Mean 
WT (PA63)7 0.70 0.85 0.78 
(PA63E302S)7 0.60 0.83 0.72 
(PA63E343S)7 0.68 0.80 0.74 
(PA63D276S)7 0.70 0.74 0.72 
(PA63D335S)7 0.58 0.77 0.68 
(PA63E308S)7 0.55 0.72 0.64 
(PA63D315S)7 0.50 0.63 0.57 
(PA63F427A)7 0.38 0.48 0.43 

Negative fixed charge density (molarity) of channels (calculated from Eq. 1) with [KCl]trans = 100 and 250 mM KCl. Each entry is the value of [N] that fits the data in Figs. 2 and 3.

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