Table 4.

Effect of mutations at position 9′ of the M2 α-helix on the time course of deactivation

Channel τdeactivation P-value Number of patches 
 (mean ± SE; ms) (range; ms)   
Wild-type nAChR 1.12 ± 0.10 0.77–1.71 1.0 × 10−15 12 
αL9′A AChR 100 ± 5 88.9–112 
Wild-type GLIC 0.91 ± 0.20 0.41–1.91 – 11 
I9′A GLICa 162 155; 169 
Wild-type ELIC 17.2 ± 3.2 7.34–26.3 – 
L9′A ELIC 5.01 ± 0.95 4.02–9.23 2.7 × 10−3 
L9′A + F16′L ELIC 5.11 ± 1.04 4.01–9.61 3.0 × 10−3 
L9′Q ELIC 15.3 ± 1.9 8.75–18.4 0.60 
L9′E ELIC 12.6 ± 0.9 10.4–15.3 0.17 
Channel τdeactivation P-value Number of patches 
 (mean ± SE; ms) (range; ms)   
Wild-type nAChR 1.12 ± 0.10 0.77–1.71 1.0 × 10−15 12 
αL9′A AChR 100 ± 5 88.9–112 
Wild-type GLIC 0.91 ± 0.20 0.41–1.91 – 11 
I9′A GLICa 162 155; 169 
Wild-type ELIC 17.2 ± 3.2 7.34–26.3 – 
L9′A ELIC 5.01 ± 0.95 4.02–9.23 2.7 × 10−3 
L9′A + F16′L ELIC 5.11 ± 1.04 4.01–9.61 3.0 × 10−3 
L9′Q ELIC 15.3 ± 1.9 8.75–18.4 0.60 
L9′E ELIC 12.6 ± 0.9 10.4–15.3 0.17 
a

High-quality recordings could only be obtained from two outside-out patches. Hence, neither the standard error nor the p-value was calculated.

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