Boltzmann-fit parameters of the voltage-dependent BK channel activation in the presence of auxiliary BKγ wild types, chimeras, and mutants
| Expression | Boltzmann fit parameters | ||
| z | n | ||
| mV | |||
| BKα alone | 167 ± 2 | 1.26 ± 0.07 | 8 |
| + γ1 | 22 ± 4 | 1.75 ± 0.09 | 7 |
| + γ2 | 61 ± 3 | 1.17 ± 0.07 | 8 |
| + γ3 | 115 ± 2 | 1.36 ± 0.05 | 6 |
| + γ4 | 154 ± 3 | 1.27 ± 0.07 | 7 |
| + γ1/γ4-signal | 13 ± 3 | 1.49 ± 0.06 | 4 |
| + γ3/γ1-signal | 121 ± 5 | 1.37 ± 0.06 | 3 |
| + γ4/γ1-signal | 155 ± 1 | 1.32 ± 0.03 | 4 |
| + γ1/γ2-LRRD | −3 ± 3 | 1.38 ± 0.04 | 6 |
| + γ1/γ3-LRRD | 6 ± 3 | 1.52 ± 0.09 | 8 |
| + γ1/γ4-LRRD | −12 ± 3 | 1.66 ± 0.05 | 11 |
| + γ1/γ2-TM&tail | 90 ± 2 | 1.28 ± 0.15 | 4 |
| + γ1/γ3-TM&tail | 151 ± 3 | 1.48 ± 0.07 | 6 |
| + γ1/γ2-tail | 143 ± 3 | 1.26 ± 0.05 | 4 |
| + γ1/γ3-tail | 25 (75%)a | 1.71 | 3 |
| 126 (25%) | 1.06 | ||
| 28 (16%) | 1.33 | 8 | |
| 150 (84%) | 0.95 | ||
| + γ1/γ4-tail | 147 ± 3 | 1.23 ± 0.08 | 5 |
| + γ2/γ1-tail | 17 ± 2 | 1.86 ± 0.10 | 3 |
| + γ2/γ3-tail | 21 ± 2 | 1.67 ± 0.12 | 4 |
| + γ2/γ4-tail | 68 ± 2 | 1.12 ± 0.08 | 5 |
| + γ3/γ1-tail | 120 ± 2 | 1.81 ± 0.20 | 3 |
| + γ3/γ2-tail | 141 ± 5 | 1.66 ± 0.20 | 3 |
| + γ3/γ4-tail | 156 ± 4 | 1.40 ± 0.07 | 3 |
| + γ1/γ2-TM | 32 ± 3 | 1.40 ± 0.07 | 5 |
| + γ1/γ3-TM | 135 ± 2 | 1.24 ± 0.02 | 3 |
| + γ1/γ4-TM | 155 ± 4 | 1.30 ± 0.03 | 5 |
| + γ2/γ1-TM | 155 ± 4 | 1.33 ± 0.09 | 6 |
| + γ2/γ3-TM | 155 ± 5 | 1.54 ± 0.07 | 4 |
| + γ2/γ4-TM | 158 ± 7 | 1.46 ± 0.10 | 4 |
| + γ3/γ1-TM | 165 ± 4 | 1.14 ± 0.05 | 5 |
| 119 | 1.11 | 1 | |
| + γ3D269R/γ1-TM | 138 ± 6 (36%) | 1.12 ± 0.10 | 7 |
| 62 ± 3 (64%) | 1.11 ± 0.05 | ||
| + γ3D269R | 117 ± 3 | 1.36 ± 0.11 | 5 |
| + γ3/γ2-TM | 26 ± 2 | 1.44 ± 0.06 | 5 |
| + γ3/γ4-TM | 160 ± 4 | 1.38 ± 0.06 | 4 |
| 113 ± 4 | 1.15 ± 0.11 | 4 | |
| + γ4/γ2-TM | 72 ± 3 | 1.27 ± 0.06 | 5 |
| + γ1-ΔtailC302–334 | 29 ± 4 | 1.40 ± 0.09 | 4 |
| + γ1-ΔtailN291–298 | 168 ± 3 | 1.22 ± 0.11 | 5 |
| + γ2-ΔtailN277–279 | 73 ± 3 | 1.12 ± 0.04 | 7 |
| + γ2-ΔtailC280–313 | 63 ± 2 | 1.28 ± 0.09 | 4 |
| + γ2-Δtail277–313 | 166 ± 6 | 1.16 ± 0.01 | 3 |
| + γ3-ΔtailC305–311 | 109 ± 3 | 1.56 ± 0.12 | 4 |
| + γ3-ΔtailN298–304 | 180 ± 3 | 1.26 ± 0.12 | 5 |
| + γ3K304N | 144 ± 2 | 1.34 ± 0.12 | 4 |
| Expression | Boltzmann fit parameters | ||
| z | n | ||
| mV | |||
| BKα alone | 167 ± 2 | 1.26 ± 0.07 | 8 |
| + γ1 | 22 ± 4 | 1.75 ± 0.09 | 7 |
| + γ2 | 61 ± 3 | 1.17 ± 0.07 | 8 |
| + γ3 | 115 ± 2 | 1.36 ± 0.05 | 6 |
| + γ4 | 154 ± 3 | 1.27 ± 0.07 | 7 |
| + γ1/γ4-signal | 13 ± 3 | 1.49 ± 0.06 | 4 |
| + γ3/γ1-signal | 121 ± 5 | 1.37 ± 0.06 | 3 |
| + γ4/γ1-signal | 155 ± 1 | 1.32 ± 0.03 | 4 |
| + γ1/γ2-LRRD | −3 ± 3 | 1.38 ± 0.04 | 6 |
| + γ1/γ3-LRRD | 6 ± 3 | 1.52 ± 0.09 | 8 |
| + γ1/γ4-LRRD | −12 ± 3 | 1.66 ± 0.05 | 11 |
| + γ1/γ2-TM&tail | 90 ± 2 | 1.28 ± 0.15 | 4 |
| + γ1/γ3-TM&tail | 151 ± 3 | 1.48 ± 0.07 | 6 |
| + γ1/γ2-tail | 143 ± 3 | 1.26 ± 0.05 | 4 |
| + γ1/γ3-tail | 25 (75%)a | 1.71 | 3 |
| 126 (25%) | 1.06 | ||
| 28 (16%) | 1.33 | 8 | |
| 150 (84%) | 0.95 | ||
| + γ1/γ4-tail | 147 ± 3 | 1.23 ± 0.08 | 5 |
| + γ2/γ1-tail | 17 ± 2 | 1.86 ± 0.10 | 3 |
| + γ2/γ3-tail | 21 ± 2 | 1.67 ± 0.12 | 4 |
| + γ2/γ4-tail | 68 ± 2 | 1.12 ± 0.08 | 5 |
| + γ3/γ1-tail | 120 ± 2 | 1.81 ± 0.20 | 3 |
| + γ3/γ2-tail | 141 ± 5 | 1.66 ± 0.20 | 3 |
| + γ3/γ4-tail | 156 ± 4 | 1.40 ± 0.07 | 3 |
| + γ1/γ2-TM | 32 ± 3 | 1.40 ± 0.07 | 5 |
| + γ1/γ3-TM | 135 ± 2 | 1.24 ± 0.02 | 3 |
| + γ1/γ4-TM | 155 ± 4 | 1.30 ± 0.03 | 5 |
| + γ2/γ1-TM | 155 ± 4 | 1.33 ± 0.09 | 6 |
| + γ2/γ3-TM | 155 ± 5 | 1.54 ± 0.07 | 4 |
| + γ2/γ4-TM | 158 ± 7 | 1.46 ± 0.10 | 4 |
| + γ3/γ1-TM | 165 ± 4 | 1.14 ± 0.05 | 5 |
| 119 | 1.11 | 1 | |
| + γ3D269R/γ1-TM | 138 ± 6 (36%) | 1.12 ± 0.10 | 7 |
| 62 ± 3 (64%) | 1.11 ± 0.05 | ||
| + γ3D269R | 117 ± 3 | 1.36 ± 0.11 | 5 |
| + γ3/γ2-TM | 26 ± 2 | 1.44 ± 0.06 | 5 |
| + γ3/γ4-TM | 160 ± 4 | 1.38 ± 0.06 | 4 |
| 113 ± 4 | 1.15 ± 0.11 | 4 | |
| + γ4/γ2-TM | 72 ± 3 | 1.27 ± 0.06 | 5 |
| + γ1-ΔtailC302–334 | 29 ± 4 | 1.40 ± 0.09 | 4 |
| + γ1-ΔtailN291–298 | 168 ± 3 | 1.22 ± 0.11 | 5 |
| + γ2-ΔtailN277–279 | 73 ± 3 | 1.12 ± 0.04 | 7 |
| + γ2-ΔtailC280–313 | 63 ± 2 | 1.28 ± 0.09 | 4 |
| + γ2-Δtail277–313 | 166 ± 6 | 1.16 ± 0.01 | 3 |
| + γ3-ΔtailC305–311 | 109 ± 3 | 1.56 ± 0.12 | 4 |
| + γ3-ΔtailN298–304 | 180 ± 3 | 1.26 ± 0.12 | 5 |
| + γ3K304N | 144 ± 2 | 1.34 ± 0.12 | 4 |
n values are the number of recorded excised inside-out patches from different HEK-293 cells.
The indicated percentage in parentheses refers to the portion of the channels’ subpopulation that was obtained from a double-Boltzmann function fit.