Conductance state in which MTS reaction occurred
| Mutant | MTS reagent | Side of addition | Conductance change (%) | Number in open state | Number in closed state |
| T301C | EA | trans | −47 ± 4 (n = 6) | 4 | 1 |
| S305C | EA | trans | −72 ± 3 (n = 21) | 25 | 5 |
| L307C | EA | trans | −65 ± 2 (n = 5) | 5 | 1 |
| G309C | ET | trans | −30 ± 3 (n = 4) | 4 | 0 |
| S312C | ET | cis | −78 ± 5 (n = 2) | 2 | 0 |
| “ | ET | trans | −75 ± 4 (n = 3) | 2 | 1 |
| A334C | EA | trans | −38 ± 2 (n = 11) | 10 | 1 |
| L338C | EA | trans | −34 ± 3 (n = 10) | 15 | 0 |
| V351C | EA | trans | −44 ± 9 (n = 3) | 1 | 2 |
| Mutant | MTS reagent | Side of addition | Conductance change (%) | Number in open state | Number in closed state |
| T301C | EA | trans | −47 ± 4 (n = 6) | 4 | 1 |
| S305C | EA | trans | −72 ± 3 (n = 21) | 25 | 5 |
| L307C | EA | trans | −65 ± 2 (n = 5) | 5 | 1 |
| G309C | ET | trans | −30 ± 3 (n = 4) | 4 | 0 |
| S312C | ET | cis | −78 ± 5 (n = 2) | 2 | 0 |
| “ | ET | trans | −75 ± 4 (n = 3) | 2 | 1 |
| A334C | EA | trans | −38 ± 2 (n = 11) | 10 | 1 |
| L338C | EA | trans | −34 ± 3 (n = 10) | 15 | 0 |
| V351C | EA | trans | −44 ± 9 (n = 3) | 1 | 2 |
The percent conductance change is given as mean ± SD, with n being the number of single-channel reactions that were used in calculating each value. All results are from experiments with single-channel resolution. Low-pass filtering was 1,000 Hz, and the sample interval was 0.15 ms. The percent conductance change and state of reaction could not always both be determined for a given channel, so the number reacting in the open state plus that in the closed state does not necessarily add up to the n value given for the conductance change. All mutant channels were tested at positive voltage (30–120 mV) with solutions as in Fig. 2.