Rate and equilibrium constant values obtained from the global fits of the J&W model to three independent single-channel data sets
| Rate and equilibrium constants | Unit | J&W |
| α1 | s−1 | 86,300 ± 100% |
| β1 | s−1 | 16 ± 64% |
| α2 | s−1 | 14,900 ± 41% |
| β2 | s−1 | 37,400 ± 93% |
| α3 | s−1 | 127 ± 13% |
| β3 | s−1 | 56,600 ± 6% |
| d−1 | s−1 | 12,400 ± 100% |
| d+1 | s−1 | 336,000 ± 99% |
| d−2 | s−1 | 4,680 ± 75% |
| d+2 | s−1 | 1,730 ± 19% |
| d−3 | s−1 | 5,460 ± 18% |
| d+3 | s−1 | 6,010 ± 20% |
| k−1 | s−1 | 378,000 ± 83% |
| k+1 | M−1 s−1 | 6.88 × 105 ± 52% |
| k−2 | s−1 | 476 ± 7% |
| k+2 | M−1 s−1 | 3.78 × 105 ± 5% |
| k−3 | s−1 | 112,000 ± 99% |
| k+3 | M−1 s−1 | 1.08 × 108 ± 99% |
| E1 = β1/α1 | 0.37 ± 99% | |
| E2 = β2/α2 | 1.5 ± 81% | |
| E3 = β3/α3 | 450 ± 8% | |
| K1 = k−1/k+1 | mM | 1,500 ± 62% |
| K2 = k−2/k+2 | mM | 1.3 ± 8% |
| K3 = k−3/k+3 | mM | 1.3 ± 12% |
| D1 = d+1/d−1 | 210 ± 74% | |
| D2 = d+2/d−2 | 0.99 ± 45% | |
| D3 = d+3/d−3 | 1.3 ± 41% | |
| EC50 | mM | 1.9 ± 43% |
| nH | 2.3 ± 10% |
| Rate and equilibrium constants | Unit | J&W |
| α1 | s−1 | 86,300 ± 100% |
| β1 | s−1 | 16 ± 64% |
| α2 | s−1 | 14,900 ± 41% |
| β2 | s−1 | 37,400 ± 93% |
| α3 | s−1 | 127 ± 13% |
| β3 | s−1 | 56,600 ± 6% |
| d−1 | s−1 | 12,400 ± 100% |
| d+1 | s−1 | 336,000 ± 99% |
| d−2 | s−1 | 4,680 ± 75% |
| d+2 | s−1 | 1,730 ± 19% |
| d−3 | s−1 | 5,460 ± 18% |
| d+3 | s−1 | 6,010 ± 20% |
| k−1 | s−1 | 378,000 ± 83% |
| k+1 | M−1 s−1 | 6.88 × 105 ± 52% |
| k−2 | s−1 | 476 ± 7% |
| k+2 | M−1 s−1 | 3.78 × 105 ± 5% |
| k−3 | s−1 | 112,000 ± 99% |
| k+3 | M−1 s−1 | 1.08 × 108 ± 99% |
| E1 = β1/α1 | 0.37 ± 99% | |
| E2 = β2/α2 | 1.5 ± 81% | |
| E3 = β3/α3 | 450 ± 8% | |
| K1 = k−1/k+1 | mM | 1,500 ± 62% |
| K2 = k−2/k+2 | mM | 1.3 ± 8% |
| K3 = k−3/k+3 | mM | 1.3 ± 12% |
| D1 = d+1/d−1 | 210 ± 74% | |
| D2 = d+2/d−2 | 0.99 ± 45% | |
| D3 = d+3/d−3 | 1.3 ± 41% | |
| EC50 | mM | 1.9 ± 43% |
| nH | 2.3 ± 10% |
All of the rate constants were fitted freely in HJCFIT. The values for efficacy at each level of ligation, E, the microscopic dissociation equilibrium constants per site, K, the equilibrium constants for the entry into the extra shut states, Dn, the EC50 and the Hill slope for the Popen curve were calculated from the rate constants for each set, and their means are listed at the bottom of the table. Values are means ± coefficient of variation (i.e., SD of the mean expressed as a percentage of the mean).