Table 1. Parameters determined in AP... | Rockefeller University Press

Table 1.

Parameters determined in AP stimulation experiments

Parameter	WT	R6/2	Significance
	APs
RT_30–70 (ms)	0.286 ± 0.022	0.49 ± 0.049	***
t_peak (ms)	1.183 ± 0.045	1.678 ± 0.091	***
t_1/2 (ms)	0.745 ± 0.022	1.10 ± 0.112	*
	Calcium signals
R	2.95 ± 0.008	2.938 ± 0.013
-ΔR_peak	1.711 ± 0.016	1.376 ± 0.022	***
τ₁ (ms)	29.69 ± 0.49	38.66 ± 0.98	***
τ₂ (ms)	512 ± 11.0	544 ± 16.9
A₂/(A₁ + A₂)	0.111 ± 0.003	0.144 ± 0.005	***
	Calcium removal
S_tot (mM)	2.16 ± 0.077	2.06 ± 0.601
k_on,S (µM⁻¹s⁻¹)	5.12 ± 0.118	11.0 ± 3.10
k_off,S (s⁻¹)	0.733 ± 0.017	1.64 ± 0.67
k_NS (s⁻¹)	9,620 ± 282	4,860 ± 216	***
	Calcium release flux
Amplitude (Ms⁻¹)	0.207 ± 0.006	0.081 ± 0.003	***
t_peak (ms)	1.98 ± 0.041	2.75 ± 0.078	***

Parameter	WT	R6/2	Significance
	APs
RT_30–70 (ms)	0.286 ± 0.022	0.49 ± 0.049	***
t_peak (ms)	1.183 ± 0.045	1.678 ± 0.091	***
t_1/2 (ms)	0.745 ± 0.022	1.10 ± 0.112	*
	Calcium signals
R	2.95 ± 0.008	2.938 ± 0.013
-ΔR_peak	1.711 ± 0.016	1.376 ± 0.022	***
τ₁ (ms)	29.69 ± 0.49	38.66 ± 0.98	***
τ₂ (ms)	512 ± 11.0	544 ± 16.9
A₂/(A₁ + A₂)	0.111 ± 0.003	0.144 ± 0.005	***
	Calcium removal
S_tot (mM)	2.16 ± 0.077	2.06 ± 0.601
k_on,S (µM⁻¹s⁻¹)	5.12 ± 0.118	11.0 ± 3.10
k_off,S (s⁻¹)	0.733 ± 0.017	1.64 ± 0.67
k_NS (s⁻¹)	9,620 ± 282	4,860 ± 216	***
	Calcium release flux
Amplitude (Ms⁻¹)	0.207 ± 0.006	0.081 ± 0.003	***
t_peak (ms)	1.98 ± 0.041	2.75 ± 0.078	***

Parameters obtained from recordings of APs and Ca²⁺ transients elicited by extracellular stimulation. See Materials and methods and Results for definitions. Numbers of experiments (WT vs. R6/2) were 39 versus 21 for AP recordings, 138 versus 101 for Ca²⁺ transients, and 116 versus 64 for Ca²⁺ removal and Ca²⁺ release flux determination. *, P < 0.05; ***, P < 0.001.

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