Comparison of nonadditive perturbation energies, evaluated using GIA and FMC, for different pairwise mutations
| Site pairs | ΔΔGGIA (±SE) | ΔΔGFMC |
| kcal | kcal | |
| A391-E395 | −0.15 (±0.56) | 9.12 |
| A391-T469 | 3.12 (±0.53) | 5.49 |
| A391-V476 | −1.33 (±0.55) | 6.04 |
| E395-T469 | −0.12 (±0.53) | 10.15 (7.5 ± 0.52) |
| E395-V476 | 0.64 (±0.65) | 12.68 |
| T469-V476 | 1.12 (±0.56) | 7.48 |
| Site pairs | ΔΔGGIA (±SE) | ΔΔGFMC |
| kcal | kcal | |
| A391-E395 | −0.15 (±0.56) | 9.12 |
| A391-T469 | 3.12 (±0.53) | 5.49 |
| A391-V476 | −1.33 (±0.55) | 6.04 |
| E395-T469 | −0.12 (±0.53) | 10.15 (7.5 ± 0.52) |
| E395-V476 | 0.64 (±0.65) | 12.68 |
| T469-V476 | 1.12 (±0.56) | 7.48 |
ΔΔGGIA for each residue pair and its standard error were calculated as described in Materials and methods. ΔΔGFMC was calculated using the Boltzmann slope and V1.2 of the G-V curves for the different mutants, reported in Yifrach and MacKinnon (2002). For the E395-T469 pair, our calculations of ΔΔGFMC using the parameters reported in Table 1 are shown in parentheses.