Median voltage of activation, VM, and the net free energy of activation of mutants of the Shaker KV channel
| Mutant | VM (±SEM) | n | ΔGnet (±SEM) |
| mV | kcal | ||
| WT | −44.7 (±1.0) | 5 | −13.6 (±0.3) |
| A391V | −46.0 (±0.9) | 5 | −14.0 (±0.3) |
| E395A | −64.6 (±0.9) | 5 | −19.6 (±0.3) |
| T469A | −55.4 (±0.8) | 3 | −16.8 (±0.2) |
| V476A | −61.0 (±0.9) | 8 | −18.5 (±0.2) |
| A391V-E395A | −66.4 (±0.9) | 10 | −20.1 (±0.3) |
| A391V-T469A | −46.4 (±0.8) | 6 | −14.1 (±0.2) |
| A391V-V476A | −66.7 (±0.8) | 12 | −20.2 (±0.2) |
| E395A-T469A | −75.7 (±0.8) | 4 | −23.0 (±0.2) |
| E395A-V476A | −78.8 (±1.4) | 4 | −23.9 (±0.4) |
| T469A-V476A | −68.0 (±1.0) | 5 | −20.6 (±0.3) |
| Mutant | VM (±SEM) | n | ΔGnet (±SEM) |
| mV | kcal | ||
| WT | −44.7 (±1.0) | 5 | −13.6 (±0.3) |
| A391V | −46.0 (±0.9) | 5 | −14.0 (±0.3) |
| E395A | −64.6 (±0.9) | 5 | −19.6 (±0.3) |
| T469A | −55.4 (±0.8) | 3 | −16.8 (±0.2) |
| V476A | −61.0 (±0.9) | 8 | −18.5 (±0.2) |
| A391V-E395A | −66.4 (±0.9) | 10 | −20.1 (±0.3) |
| A391V-T469A | −46.4 (±0.8) | 6 | −14.1 (±0.2) |
| A391V-V476A | −66.7 (±0.8) | 12 | −20.2 (±0.2) |
| E395A-T469A | −75.7 (±0.8) | 4 | −23.0 (±0.2) |
| E395A-V476A | −78.8 (±1.4) | 4 | −23.9 (±0.4) |
| T469A-V476A | −68.0 (±1.0) | 5 | −20.6 (±0.3) |
Gating currents for the mutants were measured either on a COVC or a TEV clamp set-up (bold). The VM of the normalized Q-V curve for all of the mutants (averaged from measurements performed in n oocytes) are reported along with the SEM (δVM). ΔGnet was evaluated as QmaxFVM and its standard error as QmaxFδVM.