Median voltage of activation, VM, and the net free energy of activation of mutants of the Shaker KV channel
| Mutant | VM (±SEM) | n | ΔGnet (±SEM) |
| mV | kcal | ||
| WT | −44.7 (±1.0) | 5 | −13.6 (±0.3) |
| R394A | −34.6 (±1.0) | 5 | −14.0 (±0.3) |
| E395A | −64.6 (±0.9) | 5 | −19.6 (±0.3) |
| V476A | −61.0 (±0.9) | 8 | −18.5 (±0.2) |
| Y485A | −47.9 (±0.9) | 5 | −14.5 (±0.3) |
| R394A-E395A | −51.0 (±0.9) | 6 | −15.5 (±0.3) |
| R394A-Y485A | −27.4 (±0.9) | 6 | −8.3 (±0.3) |
| E395A-Y485A | −50.5 (±1.0) | 4 | −15.3 (±0.3) |
| E395A-V476A | −78.8 (±1.4) | 4 | −23.9 (±0.4) |
| V476A-Y485A | −61.4 (±1.6) | 9 | −18.6 (±0.5) |
| R394A-E395A-Y485A | −40.0 (±1.9) | 4 | −12.1 (±0.6) |
| E395A-V476A-Y485A | −66.6 (±0.6) | 6 | −20.2 (±0.2) |
| Mutant | VM (±SEM) | n | ΔGnet (±SEM) |
| mV | kcal | ||
| WT | −44.7 (±1.0) | 5 | −13.6 (±0.3) |
| R394A | −34.6 (±1.0) | 5 | −14.0 (±0.3) |
| E395A | −64.6 (±0.9) | 5 | −19.6 (±0.3) |
| V476A | −61.0 (±0.9) | 8 | −18.5 (±0.2) |
| Y485A | −47.9 (±0.9) | 5 | −14.5 (±0.3) |
| R394A-E395A | −51.0 (±0.9) | 6 | −15.5 (±0.3) |
| R394A-Y485A | −27.4 (±0.9) | 6 | −8.3 (±0.3) |
| E395A-Y485A | −50.5 (±1.0) | 4 | −15.3 (±0.3) |
| E395A-V476A | −78.8 (±1.4) | 4 | −23.9 (±0.4) |
| V476A-Y485A | −61.4 (±1.6) | 9 | −18.6 (±0.5) |
| R394A-E395A-Y485A | −40.0 (±1.9) | 4 | −12.1 (±0.6) |
| E395A-V476A-Y485A | −66.6 (±0.6) | 6 | −20.2 (±0.2) |
Gating currents for the mutants were measured either on a COVC or a TEV clamp set-up (bold). The VM of the normalized Q-V curve, for all of the mutants (averaged from n oocytes), along with its SEM (δVM) are reported for each mutant. ΔGnet was evaluated as QmaxFVM and its standard error as QmaxFδVM. For all mutants, a Qmax of 13.2 electronic charges was used for ΔGnet calculations.