ENaC single-channel properties
| Construct | n | N | t | NPO | PO |
| min | |||||
| αK477CβV85Cγ | 8 | 1.9 ± 0.4 | 8.7 ± 0.6 | 0.74 ± 0.18 | 0.40 ± 0.06 |
| αK477CβV85Cγ + MTS-14-O4-MTS | 5 | 1.6 ± 0.2 | 9.0 ± 0.5 | 0.98 ± 0.32 | 0.60 ± 0.09 |
| αK477CβV85CγUncleaved | 5 | 1 | 6.7 ± 0.9 | 0.03 ± 0.01 | 0.03 ± 0.01 |
| αK477CβV85CγUncleaved + MTS-14-O4-MTS | 5 | 1.6 ± 0.4 | 9.3 ± 0.5 | 0.22 ± 0.11 | 0.12 ± 0.03 |
| αK477CβV85Cγ Low Li+ | 4 | 1.5 ± 0.3 | 9.8 ± 0.1 | 0.92 ± 0.15 | 0.67 ± 0.13 |
| αK477CβV85Cγ Low Li+ + MTS-14-O4-MTS | 7 | 1.9 ± 0.4 | 8.0 ± 1.0 | 0.46 ± 0.18 | 0.20 ± 0.08 |
| Construct | n | N | t | NPO | PO |
| min | |||||
| αK477CβV85Cγ | 8 | 1.9 ± 0.4 | 8.7 ± 0.6 | 0.74 ± 0.18 | 0.40 ± 0.06 |
| αK477CβV85Cγ + MTS-14-O4-MTS | 5 | 1.6 ± 0.2 | 9.0 ± 0.5 | 0.98 ± 0.32 | 0.60 ± 0.09 |
| αK477CβV85CγUncleaved | 5 | 1 | 6.7 ± 0.9 | 0.03 ± 0.01 | 0.03 ± 0.01 |
| αK477CβV85CγUncleaved + MTS-14-O4-MTS | 5 | 1.6 ± 0.4 | 9.3 ± 0.5 | 0.22 ± 0.11 | 0.12 ± 0.03 |
| αK477CβV85Cγ Low Li+ | 4 | 1.5 ± 0.3 | 9.8 ± 0.1 | 0.92 ± 0.15 | 0.67 ± 0.13 |
| αK477CβV85Cγ Low Li+ + MTS-14-O4-MTS | 7 | 1.9 ± 0.4 | 8.0 ± 1.0 | 0.46 ± 0.18 | 0.20 ± 0.08 |
Values of ENaC single-channel properties shown in Fig. 7, including the number of patches studied (n), mean number of channels per patch (N), mean duration of the experiments in minutes (t), mean product of the number of channels per patch, and the estimated open probability (NPO) and mean estimated channel open probability (PO). No analyzed patch contained more than three channels.