Table 1. cAMP and cGMP K d s for the... | Rockefeller University Press

Table 1.

cAMP and cGMP K_ds for the wild-type and CNB domain mutants

Structural motif	Mutant	cAMP K_d	cGMP K_d	K_d ratio	n
		nM	nM
	Wild type	148 ± 22	1,731 ± 264	11.7	6
β4–β5 hairpin	V282L	1,008 ± 53	8,951 ± 743	8.9	3
	T284N	342 ± 41	6,556 ± 633	19.2	3
	T284Q	731 ± 90	6,952 ± 1312	9.5	3
	T284S	464 ± 58	2,984 ± 379	6.4	6
	V288A	393 ± 50	2,544 ± 355	6.5	3
	V288L	201 ± 19	996 ± 163	5.2	3
	V288I	138 ± 39	797 ± 118	5.8	6
	V288S	1,205 ± 160	2,105 ± 128	1.7	4
αP helix	M299C	501 ± 99	2,942 ± 357	5.9	3
	M299Q	734 ± 134	11,649 ± 2,621	15.9	3
C terminus	A352S	174 ± 23	727 ± 160	4.2	3
	A352D	256 ± 29	850 ± 121	3.3	6
αC helix	R348A	24,822 ± 3182^a	38,781 ± 2,449^a	1.6	3
	R348N	19,142 ± 507^a	32,106 ± 3,844^a	1.7	3
	R348Q	25,097 ± 2,216^a	46,062 ± 5,609^a	1.8	3
	R348K	16,446 ± 842^a	23,547 ± 982^a	1.4	3
	R348Y	24,174 ± 2,214^a	33,172 ± 4,771^a	1.4	3
V288S/A352S		691 ± 72	749 ± 75	1.1	3
V288S/A352D		547 ± 111	351 ± 95	0.64	3
T284S/V288S/A352D		2,190 ± 347	336 ± 126	0.15	6

Structural motif	Mutant	cAMP K_d	cGMP K_d	K_d ratio	n
		nM	nM
	Wild type	148 ± 22	1,731 ± 264	11.7	6
β4–β5 hairpin	V282L	1,008 ± 53	8,951 ± 743	8.9	3
	T284N	342 ± 41	6,556 ± 633	19.2	3
	T284Q	731 ± 90	6,952 ± 1312	9.5	3
	T284S	464 ± 58	2,984 ± 379	6.4	6
	V288A	393 ± 50	2,544 ± 355	6.5	3
	V288L	201 ± 19	996 ± 163	5.2	3
	V288I	138 ± 39	797 ± 118	5.8	6
	V288S	1,205 ± 160	2,105 ± 128	1.7	4
αP helix	M299C	501 ± 99	2,942 ± 357	5.9	3
	M299Q	734 ± 134	11,649 ± 2,621	15.9	3
C terminus	A352S	174 ± 23	727 ± 160	4.2	3
	A352D	256 ± 29	850 ± 121	3.3	6
αC helix	R348A	24,822 ± 3182^a	38,781 ± 2,449^a	1.6	3
	R348N	19,142 ± 507^a	32,106 ± 3,844^a	1.7	3
	R348Q	25,097 ± 2,216^a	46,062 ± 5,609^a	1.8	3
	R348K	16,446 ± 842^a	23,547 ± 982^a	1.4	3
	R348Y	24,174 ± 2,214^a	33,172 ± 4,771^a	1.4	3
V288S/A352S		691 ± 72	749 ± 75	1.1	3
V288S/A352D		547 ± 111	351 ± 95	0.64	3
T284S/V288S/A352D		2,190 ± 347	336 ± 126	0.15	6

K_ds were determined by competition at a constant ligand concentration and with variable protein concentration; except for those marked with footnote a. K_d ratio is calculated from (cGMP K_d/cAMP K_d); n, number of repetitions.

K_ds were determined by competition at a constant protein concentration and with variable ligand concentration.

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