F− and Cl− turnover for CLC-pst and CLC-eve mutants
| Subclade | Homologue | F− turnover | Cl− turnover | F−/Cl− ratio |
| s−1 | s−1 | |||
| N-T → M-V | CLC-pst N82M | 1,010 ± 50 | 320 ± 20 | 2.6 ± 0.4 |
| N-T → M-V | CLC-pst T123V | 430 ± 10 | 130 ± 10 | 3.7 ± 0.8 |
| N-T → M-V | CLC-pst N82M/T123V | 1,030 ± 70 | 120 ± 10 | 9.2 ± 1.2 |
| M-V → N-T | CLC-eve M83N | 270 ± 10 | 30 ± 0 | 11.2 ± 1.2 |
| M-V → N-T | CLC-eve V124T | 310 ± 10 | 170 ± 20 | 1.9 ± 0.2 |
| M-V → N-T | CLC-eve M83N/V124T | 190 ± 10 | 70 ± 10 | 2.9 ± 0.4 |
| Subclade | Homologue | F− turnover | Cl− turnover | F−/Cl− ratio |
| s−1 | s−1 | |||
| N-T → M-V | CLC-pst N82M | 1,010 ± 50 | 320 ± 20 | 2.6 ± 0.4 |
| N-T → M-V | CLC-pst T123V | 430 ± 10 | 130 ± 10 | 3.7 ± 0.8 |
| N-T → M-V | CLC-pst N82M/T123V | 1,030 ± 70 | 120 ± 10 | 9.2 ± 1.2 |
| M-V → N-T | CLC-eve M83N | 270 ± 10 | 30 ± 0 | 11.2 ± 1.2 |
| M-V → N-T | CLC-eve V124T | 310 ± 10 | 170 ± 20 | 1.9 ± 0.2 |
| M-V → N-T | CLC-eve M83N/V124T | 190 ± 10 | 70 ± 10 | 2.9 ± 0.4 |
Turnovers on the indicated mutants were measured as described in legend to Table 2. Ratios were calculated from each set of independent trials (n = 6).