Table 3. Thermodynamic parameters for F... | Rockefeller University Press

Table 3.

Thermodynamic parameters for F⁻ binding to CLC^F homologues

Homologue	Condition	K_d	ΔH	ΔS
		mM	kJ/mol	J/mol/K
CLC-eca	Halide free	0.26	−42	−73
CLC-eca	50 mM Cl⁻ present	1.4 (12)	−38	−72
CLC-eve	50 mM Cl⁻ present	0.41	−34	−50
CLC-pst	Halide free	17	−32	−72
CLC-pst	50 mM Cl⁻ present	56 (22)	−83	−255

Homologue	Condition	K_d	ΔH	ΔS
		mM	kJ/mol	J/mol/K
CLC-eca	Halide free	0.26	−42	−73
CLC-eca	50 mM Cl⁻ present	1.4 (12)	−38	−72
CLC-eve	50 mM Cl⁻ present	0.41	−34	−50
CLC-pst	Halide free	17	−32	−72
CLC-pst	50 mM Cl⁻ present	56 (22)	−83	−255

F⁻ titration of CLC^F homologues that were amenable to the conditions required for the experiment. Parameters were obtained from a fit to a single-site binding isotherm. Values in parentheses indicate the calculated K_d for Cl⁻, assuming competitive binding.

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