Table 2.

F and Cl turnover rates for several CLCF homologues

Subclade Homologue F turnover Cl turnover F/Cl ratio for subclade 
  s−1 s−1  
N-T CLC-pst 810 ± 80 320 ± 20  
N-T CLC-rpi 420 ± 30 130 ± 10 2.6 ± 0.2 
N-T CLC-psy 460 ± 40 230 ± 10  
M-V CLC-eca 880 ± 40 280 ± 20  
M-V CLC-lla 580 ± 30 90 ± 10 6.1 ± 0.6 
M-V CLC-eve 420 ± 40 50 ± 10  
Subclade Homologue F turnover Cl turnover F/Cl ratio for subclade 
  s−1 s−1  
N-T CLC-pst 810 ± 80 320 ± 20  
N-T CLC-rpi 420 ± 30 130 ± 10 2.6 ± 0.2 
N-T CLC-psy 460 ± 40 230 ± 10  
M-V CLC-eca 880 ± 40 280 ± 20  
M-V CLC-lla 580 ± 30 90 ± 10 6.1 ± 0.6 
M-V CLC-eve 420 ± 40 50 ± 10  

F and Cl turnovers were calculated (±SEM; n = 6) using three independent reconstitutions of protein at 3 µg/mg lipid, and each preparation was assayed in duplicate. Subclade ratios were calculated from the ratios of independent trials (n = 18). The difference in the F/Cl ratio between the subclades is statistically significant (P < 0.001).

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