Summary of effects of PD on the voltage dependence of activation and inactivation for hERG1 monomers (WT) and tetramers containing zero to four L646E (LE) subunits
| Channel type | Control | 10 µM PD | n | ||
| V0.5 | z | V0.5 | z | ||
| mV | mV | ||||
| V0.5act | |||||
| WT hERG1 | −29.8 ± 0.8 | 3.41 ± 0.09 | −29.2 ± 14 | 3.07 ± 0.34 | 6 |
| WT4 | −29.1 ± 0.9 | 3.45 ± 0.33 | −26.7 ± 1.0 | 3.07 ± 0.30 | 8 |
| LE1/WT3 | −32.1 ± 1.3 | 4.13 ± 0.14 | −30.1 ± 1.6 | 4.20 ± 0.07 | 11 |
| WT3/LE1 | −29.1 ± 1.5 | 3.36 ± 0.21 | −27.1 ± 1.29 | 3.44 ± 0.16 | 4 |
| LE1/WT1/LE1/WT1 | −36.7 ± 0.5 | 3.23 ± 0.08 | −32.7 ± 0.8 | 3.45 ± 0.19 | 6 |
| LE2/WT2 | −35.4 ± 0.8 | 3.88 ± 0.12 | −31.3 ± 1.0 | 3.94 ± 0.12 | 12 |
| LE1/WT1/LE2 | −33.5 ± 1.6 | 3.41 ± 0.18 | −30.3 ± 1.4 | 3.55 ± 0.15 | 6 |
| LE4 | −36.5 ± 1.0 | 3.55 ± 0.20 | −32.6 ± 1.2 | 3.55 ± 0.25 | 9 |
| V0.5inact | |||||
| WT hERG1 | −55.8 ± 3.7 | 1.19 ± 0.02 | −34.7 ± 4.2 | 1.19 ± 0.09 | 5 |
| WT4 | −55.7 ± 0.7 | 1.10 ± 0.02 | −39.4 ± 4.6 | 1.26 ± 0.17 | 4 |
| LE1/WT3 | −48.5 ± 2.7 | 1.02 ± 0.05 | −44.6 ± 2.7 | 1.24 ± 0.10 | 8 |
| WT3/LE1 | −37.4 ± 3.0 | 1.23 ± 0.02 | −33.5 ± 3.7 | 1.44 ± 0.19 | 4 |
| LE1/WT1/LE1/WT1 | −42.6 ± 3.6 | 1.08 ± 0.05 | −43.3 ± 3.4 | 1.22 ± 0.07 | 5 |
| LE2/WT2 | −45.7 ± 1.9 | 1.07 ± 0.03 | −45.5 ± 2.8 | 1.19 ± 0.08 | 7 |
| LE1/WT1/LE2 | −44.3 ± 5.5 | 1.27 ± 0.08 | −47.7 ± 4.0 | 1.31 ± 0.08 | 4 |
| LE4 | −41.4 ± 1.6 | 1.49 ± 0.04 | −43.6 ± 2.2 | 1.52 ± 0.05 | 6 |
| Channel type | Control | 10 µM PD | n | ||
| V0.5 | z | V0.5 | z | ||
| mV | mV | ||||
| V0.5act | |||||
| WT hERG1 | −29.8 ± 0.8 | 3.41 ± 0.09 | −29.2 ± 14 | 3.07 ± 0.34 | 6 |
| WT4 | −29.1 ± 0.9 | 3.45 ± 0.33 | −26.7 ± 1.0 | 3.07 ± 0.30 | 8 |
| LE1/WT3 | −32.1 ± 1.3 | 4.13 ± 0.14 | −30.1 ± 1.6 | 4.20 ± 0.07 | 11 |
| WT3/LE1 | −29.1 ± 1.5 | 3.36 ± 0.21 | −27.1 ± 1.29 | 3.44 ± 0.16 | 4 |
| LE1/WT1/LE1/WT1 | −36.7 ± 0.5 | 3.23 ± 0.08 | −32.7 ± 0.8 | 3.45 ± 0.19 | 6 |
| LE2/WT2 | −35.4 ± 0.8 | 3.88 ± 0.12 | −31.3 ± 1.0 | 3.94 ± 0.12 | 12 |
| LE1/WT1/LE2 | −33.5 ± 1.6 | 3.41 ± 0.18 | −30.3 ± 1.4 | 3.55 ± 0.15 | 6 |
| LE4 | −36.5 ± 1.0 | 3.55 ± 0.20 | −32.6 ± 1.2 | 3.55 ± 0.25 | 9 |
| V0.5inact | |||||
| WT hERG1 | −55.8 ± 3.7 | 1.19 ± 0.02 | −34.7 ± 4.2 | 1.19 ± 0.09 | 5 |
| WT4 | −55.7 ± 0.7 | 1.10 ± 0.02 | −39.4 ± 4.6 | 1.26 ± 0.17 | 4 |
| LE1/WT3 | −48.5 ± 2.7 | 1.02 ± 0.05 | −44.6 ± 2.7 | 1.24 ± 0.10 | 8 |
| WT3/LE1 | −37.4 ± 3.0 | 1.23 ± 0.02 | −33.5 ± 3.7 | 1.44 ± 0.19 | 4 |
| LE1/WT1/LE1/WT1 | −42.6 ± 3.6 | 1.08 ± 0.05 | −43.3 ± 3.4 | 1.22 ± 0.07 | 5 |
| LE2/WT2 | −45.7 ± 1.9 | 1.07 ± 0.03 | −45.5 ± 2.8 | 1.19 ± 0.08 | 7 |
| LE1/WT1/LE2 | −44.3 ± 5.5 | 1.27 ± 0.08 | −47.7 ± 4.0 | 1.31 ± 0.08 | 4 |
| LE4 | −41.4 ± 1.6 | 1.49 ± 0.04 | −43.6 ± 2.2 | 1.52 ± 0.05 | 6 |
Data are expressed as mean ± SEM (n = number of oocytes).