CG-MALS, globally fitted parameters at saturating Ca2+
| Sample | Fitted molar massa | Fitted incompetent fractionb | Fitted log (KA) | Binding site Kd |
| kD | log (1/M) | nM | ||
| SKp | 11.5 (11.2–11.5) | 0.130 (0–0.13) | ||
| CaM | 16.4 (16.4–16.6) | 0.101 (0–0.10) | ||
| (SKp)(CaM) | 27.9 | 10.2 (9.9–10.9) | 0.06 (0.01–0.13) | |
| (SKp)2(CaM) | 39.4 | 16.9 (16.3–17.6) | 194 (190–360) | |
| (SKp)(CaM)2 | 44.3 | 16.0 (15.5–16.5) | 1,750 (1,700–2,800) |
| Sample | Fitted molar massa | Fitted incompetent fractionb | Fitted log (KA) | Binding site Kd |
| kD | log (1/M) | nM | ||
| SKp | 11.5 (11.2–11.5) | 0.130 (0–0.13) | ||
| CaM | 16.4 (16.4–16.6) | 0.101 (0–0.10) | ||
| (SKp)(CaM) | 27.9 | 10.2 (9.9–10.9) | 0.06 (0.01–0.13) | |
| (SKp)2(CaM) | 39.4 | 16.9 (16.3–17.6) | 194 (190–360) | |
| (SKp)(CaM)2 | 44.3 | 16.0 (15.5–16.5) | 1,750 (1,700–2,800) |
Parameters were determined from globally fitted data; however, values in parenthesis are ranges of parameters determined from fitted subsets of data. The ranges were determined by floating or fixing SKp and CaM molar masses, allowing for various fitted incompetent fractions, and adding additional stoichiometries, e.g., 2SKp/2CaM complex. The χ2 values from fitting subsets of data or global data are χ2 < 0.9.
The molar masses of SKp and CaM were fitted and the masses of protein complexes were computed by summation.
To show that solutions in this table are tightly constrained, some fits were performed with incompetent fractions fixed at zero, which only slightly broadened the ranges of the other parameters.