CG-MALS, globally fitted parameters at zero Ca2+
| Sample | Molar massa | Fitted incompetent fractionb | Fitted log (KA) | Binding site Kd |
| kD | log (1/M) | nM | ||
| SKp | 11.4 (11.3–11.5) | 0.153 (0.11–0.25) | ||
| CaM | 16.7 (16.5–16.9) | 0.082 (0.01–0.18) | ||
| (SKp)(CaM) | 28.1 | 9.26 (7.3–10.1) | 0.6 (0.08–2.8) | |
| (SKp)2(CaM) | 39.5 | 14.4 (12.2–15.8) | 8,000 (5,000–12,000) |
| Sample | Molar massa | Fitted incompetent fractionb | Fitted log (KA) | Binding site Kd |
| kD | log (1/M) | nM | ||
| SKp | 11.4 (11.3–11.5) | 0.153 (0.11–0.25) | ||
| CaM | 16.7 (16.5–16.9) | 0.082 (0.01–0.18) | ||
| (SKp)(CaM) | 28.1 | 9.26 (7.3–10.1) | 0.6 (0.08–2.8) | |
| (SKp)2(CaM) | 39.5 | 14.4 (12.2–15.8) | 8,000 (5,000–12,000) |
Parameters are determined from globally fitted data; however, values in parenthesis are ranges of parameters determined from fitted subsets of data. The ranges were determined by floating or fixing SKp and CaM molar masses, allowing for various fitted incompetent fractions, and adding additional stoichiometries, e.g., 1SKp/2CaM complex or 2SKp/2CaM complex. The χ2 values from fitting subsets of data or global data ranged from 0.02 < χ2 < 0.1.
The molar masses of SKp and CaM were fitted and the masses of protein complexes were computed by summation.
Because data in this table are not tightly constrained, solutions were ignored that result in >30% incompetent fraction yield based on empirical observations that we do not have a large population of inactive protein.