Table 5. Resultant parameters by fitting... | Rockefeller University Press

Table 5.

Resultant parameters by fitting to TRPC currents with SPD model

Cell		HEK293	HEK293	A7r5	HEK293
Transfected plasmids		TRPC6/CFPmse-PHd/YFPmse-PHd	TRPC6/M₁R/CFPmse-PHd/YFPmse-PHd	CFPmse-PHd/YFPmse-PHd	TRPC7-CFPmse/M₁R/YFPmse-PHd
Receptor agonist (conc. µM)		CCh (100)	CCh (100)	Arg-Vasopressin (1)	CCh (100)
The number of fitted cells		4	4	4	4
Row	Parameters (unit)
1	PI(4,5)P₂ (µM) at resting	22.5 ± 0.4	23.0 ± 0.4	22.2 ± 0. 2	14.6 ± 0.1
2	k_i_PLC (s⁻¹)	0.05 ± 0.02	1.2 ± 0.20	1.3 ± 0.42	0.93 ± 0.09
3	k_ii_DAG kinase (s⁻¹)	0.04 ± 0.01	0.03 ± 0.02	0.07 ± 0.03	0.02 ± 0.01
4	k_iii_PA to PIP reactions, (s⁻¹)	0.008 ± 0.002	0.006 ± 0.002	0.004 ± 0.001	0.006 ± 0.0001
5	k_iv_PIP5K (s⁻¹)	0.050 ± 0.004	0.07 ± 0.04	0.17 ± 0.083	0.04 ± 0.03
6	k_v_IP₃ phosphatase (s⁻¹)	0.7 ± 0.4	0.2 ± 0.1	0.88 ± 0.093	0.84 ± 0.14
7	τ_rd (s)	12.0 ± 3.8	1.4 ± 0.6	1.4 ± 0.2	4.4 ± 0.4
8	τ_sd (s)	24 ± 11	17 ± 5.2	6 ± 2.4	38 ± 15
9	Rd_f (no unit)	0.65 ± 0.08	0.55 ± 0.03	0.60 ± 0.12	0.46 ± 0.05
10	Sd_f (no unit)	0.35 ± 0.05	0.44 ± 0.02	0.43 ± 0.124	0.53 ± 0.06
11	spot (distance global to local; µm)	0.64 ± 0.09	2.78 ± 0.79	2.83 ± 0.98	1.90 ± 0.29
12	dcoef of PI(4,5)P₂ (µm²/s)	0.36 ± 0.08	0.8 ± 0.47	0.5 ± 0.11	0.29 ± 0.06
13	Ratio of local k_i / global k_i	1.1 ± 0.28	4.0 ± 1.6	2.8 ± 0.98	6.0 ± 1.3
14	Activation delay factor	0.03 ± 0.024	0.01 ± 0.002	0.003 ± 0.002	0.03 ± 0.025
15	Activation power factor	0.6 ± 0.33	0.32 ± 0.07	0.7 ± 0.17	0.3 ± 0.12
16	The number of channels	1,644 ± 277	5,808 ± 959	317 ± 121	2,384 ± 1,896
17	K₁ (k_d for DAG1; µM)	39 ± 6.3	26 ± 2.4	23 ± 7.6	7.0 ± 0.3
18	K₂ (k_d for DAG2; µM)	29 ± 5.1	22 ± 2.0	8 ± 3.4	6.3 ± 0.25
19	K₃ (k_d for PI(4,5)P₂; µM)	1.9 ± 0.6	3.0 ± 0.66	9 ± 1.5	9.7 ± 0.14
	SD	0.04	0.05	0.12	0.14
	Sr_0.01^a	82.7%	80.9%	70.1%	56.2%

Cell		HEK293	HEK293	A7r5	HEK293
Transfected plasmids		TRPC6/CFPmse-PHd/YFPmse-PHd	TRPC6/M₁R/CFPmse-PHd/YFPmse-PHd	CFPmse-PHd/YFPmse-PHd	TRPC7-CFPmse/M₁R/YFPmse-PHd
Receptor agonist (conc. µM)		CCh (100)	CCh (100)	Arg-Vasopressin (1)	CCh (100)
The number of fitted cells		4	4	4	4
Row	Parameters (unit)
1	PI(4,5)P₂ (µM) at resting	22.5 ± 0.4	23.0 ± 0.4	22.2 ± 0. 2	14.6 ± 0.1
2	k_i_PLC (s⁻¹)	0.05 ± 0.02	1.2 ± 0.20	1.3 ± 0.42	0.93 ± 0.09
3	k_ii_DAG kinase (s⁻¹)	0.04 ± 0.01	0.03 ± 0.02	0.07 ± 0.03	0.02 ± 0.01
4	k_iii_PA to PIP reactions, (s⁻¹)	0.008 ± 0.002	0.006 ± 0.002	0.004 ± 0.001	0.006 ± 0.0001
5	k_iv_PIP5K (s⁻¹)	0.050 ± 0.004	0.07 ± 0.04	0.17 ± 0.083	0.04 ± 0.03
6	k_v_IP₃ phosphatase (s⁻¹)	0.7 ± 0.4	0.2 ± 0.1	0.88 ± 0.093	0.84 ± 0.14
7	τ_rd (s)	12.0 ± 3.8	1.4 ± 0.6	1.4 ± 0.2	4.4 ± 0.4
8	τ_sd (s)	24 ± 11	17 ± 5.2	6 ± 2.4	38 ± 15
9	Rd_f (no unit)	0.65 ± 0.08	0.55 ± 0.03	0.60 ± 0.12	0.46 ± 0.05
10	Sd_f (no unit)	0.35 ± 0.05	0.44 ± 0.02	0.43 ± 0.124	0.53 ± 0.06
11	spot (distance global to local; µm)	0.64 ± 0.09	2.78 ± 0.79	2.83 ± 0.98	1.90 ± 0.29
12	dcoef of PI(4,5)P₂ (µm²/s)	0.36 ± 0.08	0.8 ± 0.47	0.5 ± 0.11	0.29 ± 0.06
13	Ratio of local k_i / global k_i	1.1 ± 0.28	4.0 ± 1.6	2.8 ± 0.98	6.0 ± 1.3
14	Activation delay factor	0.03 ± 0.024	0.01 ± 0.002	0.003 ± 0.002	0.03 ± 0.025
15	Activation power factor	0.6 ± 0.33	0.32 ± 0.07	0.7 ± 0.17	0.3 ± 0.12
16	The number of channels	1,644 ± 277	5,808 ± 959	317 ± 121	2,384 ± 1,896
17	K₁ (k_d for DAG1; µM)	39 ± 6.3	26 ± 2.4	23 ± 7.6	7.0 ± 0.3
18	K₂ (k_d for DAG2; µM)	29 ± 5.1	22 ± 2.0	8 ± 3.4	6.3 ± 0.25
19	K₃ (k_d for PI(4,5)P₂; µM)	1.9 ± 0.6	3.0 ± 0.66	9 ± 1.5	9.7 ± 0.14
	SD	0.04	0.05	0.12	0.14
	Sr_0.01a	82.7%	80.9%	70.1%	56.2%

Parameters are presented as mean ± SEM. (Typical results are displayed in Fig. 7.)

Percentage of the squared residuals lower than the value of 0.01.

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