Resultant parameters by fitting to TRPC currents with SPD model
| Cell | HEK293 | HEK293 | A7r5 | HEK293 | |
| Transfected plasmids | TRPC6/CFPmse-PHd/YFPmse-PHd | TRPC6/M1R/CFPmse-PHd/YFPmse-PHd | CFPmse-PHd/YFPmse-PHd | TRPC7-CFPmse/M1R/YFPmse-PHd | |
| Receptor agonist (conc. µM) | CCh (100) | CCh (100) | Arg-Vasopressin (1) | CCh (100) | |
| The number of fitted cells | 4 | 4 | 4 | 4 | |
| Row | Parameters (unit) | ||||
| 1 | PI(4,5)P2 (µM) at resting | 22.5 ± 0.4 | 23.0 ± 0.4 | 22.2 ± 0. 2 | 14.6 ± 0.1 |
| 2 | ki_PLC (s−1) | 0.05 ± 0.02 | 1.2 ± 0.20 | 1.3 ± 0.42 | 0.93 ± 0.09 |
| 3 | kii_DAG kinase (s−1) | 0.04 ± 0.01 | 0.03 ± 0.02 | 0.07 ± 0.03 | 0.02 ± 0.01 |
| 4 | kiii_PA to PIP reactions, (s−1) | 0.008 ± 0.002 | 0.006 ± 0.002 | 0.004 ± 0.001 | 0.006 ± 0.0001 |
| 5 | kiv_PIP5K (s−1) | 0.050 ± 0.004 | 0.07 ± 0.04 | 0.17 ± 0.083 | 0.04 ± 0.03 |
| 6 | kv_IP3 phosphatase (s−1) | 0.7 ± 0.4 | 0.2 ± 0.1 | 0.88 ± 0.093 | 0.84 ± 0.14 |
| 7 | τrd (s) | 12.0 ± 3.8 | 1.4 ± 0.6 | 1.4 ± 0.2 | 4.4 ± 0.4 |
| 8 | τsd (s) | 24 ± 11 | 17 ± 5.2 | 6 ± 2.4 | 38 ± 15 |
| 9 | Rd_f (no unit) | 0.65 ± 0.08 | 0.55 ± 0.03 | 0.60 ± 0.12 | 0.46 ± 0.05 |
| 10 | Sd_f (no unit) | 0.35 ± 0.05 | 0.44 ± 0.02 | 0.43 ± 0.124 | 0.53 ± 0.06 |
| 11 | spot (distance global to local; µm) | 0.64 ± 0.09 | 2.78 ± 0.79 | 2.83 ± 0.98 | 1.90 ± 0.29 |
| 12 | dcoef of PI(4,5)P2 (µm2/s) | 0.36 ± 0.08 | 0.8 ± 0.47 | 0.5 ± 0.11 | 0.29 ± 0.06 |
| 13 | Ratio of local ki / global ki | 1.1 ± 0.28 | 4.0 ± 1.6 | 2.8 ± 0.98 | 6.0 ± 1.3 |
| 14 | Activation delay factor | 0.03 ± 0.024 | 0.01 ± 0.002 | 0.003 ± 0.002 | 0.03 ± 0.025 |
| 15 | Activation power factor | 0.6 ± 0.33 | 0.32 ± 0.07 | 0.7 ± 0.17 | 0.3 ± 0.12 |
| 16 | The number of channels | 1,644 ± 277 | 5,808 ± 959 | 317 ± 121 | 2,384 ± 1,896 |
| 17 | K1 (kd for DAG1; µM) | 39 ± 6.3 | 26 ± 2.4 | 23 ± 7.6 | 7.0 ± 0.3 |
| 18 | K2 (kd for DAG2; µM) | 29 ± 5.1 | 22 ± 2.0 | 8 ± 3.4 | 6.3 ± 0.25 |
| 19 | K3 (kd for PI(4,5)P2; µM) | 1.9 ± 0.6 | 3.0 ± 0.66 | 9 ± 1.5 | 9.7 ± 0.14 |
| SD | 0.04 | 0.05 | 0.12 | 0.14 | |
| Sr0.01a | 82.7% | 80.9% | 70.1% | 56.2% | |
| Cell | HEK293 | HEK293 | A7r5 | HEK293 | |
| Transfected plasmids | TRPC6/CFPmse-PHd/YFPmse-PHd | TRPC6/M1R/CFPmse-PHd/YFPmse-PHd | CFPmse-PHd/YFPmse-PHd | TRPC7-CFPmse/M1R/YFPmse-PHd | |
| Receptor agonist (conc. µM) | CCh (100) | CCh (100) | Arg-Vasopressin (1) | CCh (100) | |
| The number of fitted cells | 4 | 4 | 4 | 4 | |
| Row | Parameters (unit) | ||||
| 1 | PI(4,5)P2 (µM) at resting | 22.5 ± 0.4 | 23.0 ± 0.4 | 22.2 ± 0. 2 | 14.6 ± 0.1 |
| 2 | ki_PLC (s−1) | 0.05 ± 0.02 | 1.2 ± 0.20 | 1.3 ± 0.42 | 0.93 ± 0.09 |
| 3 | kii_DAG kinase (s−1) | 0.04 ± 0.01 | 0.03 ± 0.02 | 0.07 ± 0.03 | 0.02 ± 0.01 |
| 4 | kiii_PA to PIP reactions, (s−1) | 0.008 ± 0.002 | 0.006 ± 0.002 | 0.004 ± 0.001 | 0.006 ± 0.0001 |
| 5 | kiv_PIP5K (s−1) | 0.050 ± 0.004 | 0.07 ± 0.04 | 0.17 ± 0.083 | 0.04 ± 0.03 |
| 6 | kv_IP3 phosphatase (s−1) | 0.7 ± 0.4 | 0.2 ± 0.1 | 0.88 ± 0.093 | 0.84 ± 0.14 |
| 7 | τrd (s) | 12.0 ± 3.8 | 1.4 ± 0.6 | 1.4 ± 0.2 | 4.4 ± 0.4 |
| 8 | τsd (s) | 24 ± 11 | 17 ± 5.2 | 6 ± 2.4 | 38 ± 15 |
| 9 | Rd_f (no unit) | 0.65 ± 0.08 | 0.55 ± 0.03 | 0.60 ± 0.12 | 0.46 ± 0.05 |
| 10 | Sd_f (no unit) | 0.35 ± 0.05 | 0.44 ± 0.02 | 0.43 ± 0.124 | 0.53 ± 0.06 |
| 11 | spot (distance global to local; µm) | 0.64 ± 0.09 | 2.78 ± 0.79 | 2.83 ± 0.98 | 1.90 ± 0.29 |
| 12 | dcoef of PI(4,5)P2 (µm2/s) | 0.36 ± 0.08 | 0.8 ± 0.47 | 0.5 ± 0.11 | 0.29 ± 0.06 |
| 13 | Ratio of local ki / global ki | 1.1 ± 0.28 | 4.0 ± 1.6 | 2.8 ± 0.98 | 6.0 ± 1.3 |
| 14 | Activation delay factor | 0.03 ± 0.024 | 0.01 ± 0.002 | 0.003 ± 0.002 | 0.03 ± 0.025 |
| 15 | Activation power factor | 0.6 ± 0.33 | 0.32 ± 0.07 | 0.7 ± 0.17 | 0.3 ± 0.12 |
| 16 | The number of channels | 1,644 ± 277 | 5,808 ± 959 | 317 ± 121 | 2,384 ± 1,896 |
| 17 | K1 (kd for DAG1; µM) | 39 ± 6.3 | 26 ± 2.4 | 23 ± 7.6 | 7.0 ± 0.3 |
| 18 | K2 (kd for DAG2; µM) | 29 ± 5.1 | 22 ± 2.0 | 8 ± 3.4 | 6.3 ± 0.25 |
| 19 | K3 (kd for PI(4,5)P2; µM) | 1.9 ± 0.6 | 3.0 ± 0.66 | 9 ± 1.5 | 9.7 ± 0.14 |
| SD | 0.04 | 0.05 | 0.12 | 0.14 | |
| Sr0.01a | 82.7% | 80.9% | 70.1% | 56.2% | |
Parameters are presented as mean ± SEM. (Typical results are displayed in Fig. 7.)
Percentage of the squared residuals lower than the value of 0.01.