Table 3. Equations for the... | Rockefeller University Press

Table 3.

Equations for the back-calculated FR

Comment to the equation	Equation	No.
Fraction of IP₃ bound PHd (PHd):	$d ({PHd}_{IP3b}) = 1 - d {[PHd]}_{IP3} / {[PHd]}_{tot}$	21
Concentration of membrane bound PHd:	$d {[PHd]}_{rem} = {[PHd]}_{tot} - d {[PHd]}_{rel}$	22
Concentration of cytosolic PHd upon PI(4,5)P₂ reduction:	$d {[PHd]}_{rel} = (1 - 1 / (K_{d,PHd} / d {[PI (4, 5) P_{2}]}_{tot} + 1)) \cdot {[PHd]}_{tot}$	23
Concentration of IP₃ bound PHd:	$d {[PHd]}_{IP3} = (1 / (K_{d,IP3} / d [{IP}_{3}] + 1)) \cdot d {[PHd]}_{rel}$	24
Norm.FR between TRPC7-CFP_mse and YFP_mse-PHd:	$\begin{array}{l} Norm. FR = d ({PHd}_{IP3b}) \cdot ((1 / (1 + K_{d,PHd} / d {[PI (4, 5) P_{2}]}_{tot})) \cdot {[1 / (1 + K_{d,PHd} / {[PI (4, 5) P_{2}]}_{tot (0)})]}^{- 1}) \\ {[PI (4, 5) P_{2}]}_{tot (0)} : PI (4, 5) P_{2} concentration at time zero \end{array}$	25
Norm.FR between CFP_mse-PHd and YFP_mse-PHd:	$Norm. FR = d ({PHd}_{IP3b}) \cdot ({(1 / (1 + K_{d,PHd} / d {[PI (4, 5) P_{2}]}_{tot}))}^{2} \cdot {[{(1 / (1 + K_{d,PHd} / {[PI (4, 5) P_{2}]}_{tot (0)}))}^{2}]}^{- 1})$	26

Comment to the equation	Equation	No.
Fraction of IP₃ bound PHd (PHd):	$d ({PHd}_{IP3b}) = 1 - d {[PHd]}_{IP3} / {[PHd]}_{tot}$	21
Concentration of membrane bound PHd:	$d {[PHd]}_{rem} = {[PHd]}_{tot} - d {[PHd]}_{rel}$	22
Concentration of cytosolic PHd upon PI(4,5)P₂ reduction:	$d {[PHd]}_{rel} = (1 - 1 / (K_{d,PHd} / d {[PI (4, 5) P_{2}]}_{tot} + 1)) \cdot {[PHd]}_{tot}$	23
Concentration of IP₃ bound PHd:	$d {[PHd]}_{IP3} = (1 / (K_{d,IP3} / d [{IP}_{3}] + 1)) \cdot d {[PHd]}_{rel}$	24
Norm.FR between TRPC7-CFP_mse and YFP_mse-PHd:	$\begin{array}{l} Norm. FR = d ({PHd}_{IP3b}) \cdot ((1 / (1 + K_{d,PHd} / d {[PI (4, 5) P_{2}]}_{tot})) \cdot {[1 / (1 + K_{d,PHd} / {[PI (4, 5) P_{2}]}_{tot (0)})]}^{- 1}) \\ {[PI (4, 5) P_{2}]}_{tot (0)} : PI (4, 5) P_{2} concentration at time zero \end{array}$	25
Norm.FR between CFP_mse-PHd and YFP_mse-PHd:	$Norm. FR = d ({PHd}_{IP3b}) \cdot ({(1 / (1 + K_{d,PHd} / d {[PI (4, 5) P_{2}]}_{tot}))}^{2} \cdot {[{(1 / (1 + K_{d,PHd} / {[PI (4, 5) P_{2}]}_{tot (0)}))}^{2}]}^{- 1})$	26