Table 4. Allosteric model parameters for... | Rockefeller University Press

Table 4.

Allosteric model parameters for I696X and W697X mutants in the presence of 1 and 100 µM capsaicin

Channel	1 µM			100 µM
	D	P	ΔG_o	D	P	ΔG_o
			kcal/mol			kcal/mol
TRPV1	370	3,000	−1.45	NA	NA	NA
I696A	1,400	32	0.40	1,300	32	0.45
I696V	1,008	460	−0.78	144	460	0.33
I696L	131	1,800	−0.46	38	1,800	0.25
I696M	373	480	−0.21	134	480	0.38
I696H	504	720	−0.76	61	720	0.46
W697Y	30	2,200	0.17	50	9,500	−0.96
W697H	30	4,000	−0.15	18	7,200	−0.21
W697V	18	2,900	0.31	25	6,100	−0.31
W697D	10	13,000	−0.25	8	2,300	0.92
W697N	10	8,500	0.03	6	6,300	0.50

Channel	1 µM			100 µM
	D	P	ΔG_o	D	P	ΔG_o
			kcal/mol			kcal/mol
TRPV1	370	3,000	−1.45	NA	NA	NA
I696A	1,400	32	0.40	1,300	32	0.45
I696V	1,008	460	−0.78	144	460	0.33
I696L	131	1,800	−0.46	38	1,800	0.25
I696M	373	480	−0.21	134	480	0.38
I696H	504	720	−0.76	61	720	0.46
W697Y	30	2,200	0.17	50	9,500	−0.96
W697H	30	4,000	−0.15	18	7,200	−0.21
W697V	18	2,900	0.31	25	6,100	−0.31
W697D	10	13,000	−0.25	8	2,300	0.92
W697N	10	8,500	0.03	6	6,300	0.50

Normalized g-V curves in the presence of capsaicin were fitted to Eq. 3. For I696X and wild type, the values for the equilibrium constants were fixed to those obtained in the absence of capsaicin: J_o = 0.021, L = 5.0 × 10⁻⁴, K_d = 10⁻⁵, and z_g = 0.6. For W697X mutants, these values were the same, except for z_g = 0.80, which was taken from the Boltzmann fits. The value of E given by the analysis for all mutants was virtually 1 and, therefore, was kept constant to this value. NA, not applicable.

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