Table 1.

Pore-forming subunits of ion channels experimentally determined to be S-acylated

Channel Subunit Gene Candidate S-acylation sites UniProt ID References 
Ligand-gated      
AMPA GluA1 Gria1 593FSLGAFMQQGCDISPRSLSGRI P23818 Hayashi et al., 2005  
   819LAMLVALIEFCYKSRSESKRMK P23818 Hayashi et al., 2005  
 GluA2 Gria2 600FSLGAFMRQGCDISPRSLSGRI P23819 Hayashi et al., 2005  
   826LAMLVALIEFCYKSRAEAKRMK P23819 Hayashi et al., 2005  
 GluA3 Gria3 605FSLGAFMQQGCDISPRSLSGRI Q9Z2W9 Hayashi et al., 2005  
   831LAMMVALIEFCYKSRAESKRMK Q9Z2W9 Hayashi et al., 2005  
 GluA4 Gria4 601FSLGAFMQQGCDISPRSLSGRI Q9Z2W8 Hayashi et al., 2005  
   827LAMLVALIEFCYKSRAEAKRMK Q9Z2W8 Hayashi et al., 2005  
GABAA γ2 Gabrg2 405QERDEEYGYECLDGKDCASFFCCFEDCRTGAWRHGRI P22723 Rathenberg et al., 2004; Fang et al., 2006  
Kainate GluK2 Grik2 848KNAQLEKRSFCSAMVEELRMSLKCQRRLKHKPQAPV P39087 Pickering et al., 1995  
nAChR α4 Chrna4 263TVLVFYLPSECGEKVTLCISV O70174 Alexander et al., 2010; Amici et al., 2012  
 α7 Chrna7 ND  Alexander et al., 2010; Drisdel et al., 2004  
 β2 Chrnb2 ND  Alexander et al., 2010  
NMDA GluN2A Grin2a 838EHLFYWKLRFCFTGVCSDRPGLLFSISRGIYSCIHGVHIEEKK P35436 Hayashi et al., 2009  
   1204SDRYRQNSTHCRSCLSNLPTYSGHFTMRSPFKCDACLRMGNLYDID P35436 Hayashi et al., 2009  
 GluN2B Grin2b 839EHLFYWQFRHCFMGVCSGKPGMVFSISRGIYSCIHGVAIEERQ Q01097 Hayashi et al., 2009  
   1205DWEDRSGGNFCRSCPSKLHNYSSTVAGQNSGRQACIRCEACKKAGNLYDIS Q01097 Hayashi et al., 2009  
P2X7 P2X7 P2rx7 361AFCRSGVYPYCKCCEPCTVNEYYYRKK Q9Z1M0 Gonnord et al., 2009  
   469APKSGDSPSWCQCGNCLPSRLPEQRR Q9Z1M0 Gonnord et al., 2009  
   488PEQRRALEELCCRRKPGRCITT Q9Z1M0 Gonnord et al., 2009  
   562DMADFAILPSCCRWRIRKEFPK Q9Z1M0 Gonnord et al., 2009  
Voltage gated      
Potassium      
BK, maxiK KCa1.1 Kcnma1 43WRTLKYLWTVCCHCGGKTKEAQKI Q08460 Jeffries et al., 2010  
   635MSIYKRMRRACCFDCGRSERDCSCM Q08460 Tian et al., 2008; 2010  
 Kv1.1 Kcna1 233SFELVVRFFACPSKTDFFKNI P16388 Gubitosi-Klug et al., 2005  
 Kv1.5 Kcna5 16LRGGGEAGASCVQSPRGECGC Q61762 Jindal et al., 2008  
   583VDLRRSLYALCLDTSRETDL-stop Q61762 Zhang et al., 2007; Jindal et al., 2008  
Sodium NaV1.2 Scn2a1 ND  Schmidt and Catterall, 1987  
   640MNGKMHSAVDCNGVVSLVGGP P04775 Bosmans et al., 2011  
   1042LEDLNNKKDSCISNHTTIEIG P04775 Bosmans et al., 2011  
   1172TEDCVRKFKCCQISIEEGKGK P04775 Bosmans et al., 2011  
Other channels      
Aquaporin AQP4 Aqp4 3DRAAARRWGKCGHSCSRESIMVAFK P55088 Crane and Verkman, 2009; Suzuki et al., 2008  
CFTR CFTR CFTR 514EYRYRSVIKACQLEEDISKFAEKD P13569 McClure et al., 2012  
   1385RRTLKQAFADCTVILCEHRIEA P13569 McClure et al., 2012  
Connexin Cx32 Gjb1 270GAGLAEKSDRCSAC-stop P28230 Locke et al., 2006  
ENaC ENaC β Scnn1b 33TNTHGPKRIICEGPKKKAMWFL Q9WU38 Mueller et al., 2010  
   547WITIIKLVASCKGLRRRRPQAPY Q9WU38 Mueller et al., 2010  
 ENaC γ Scnn1g 23PTIKDLMHWYCLNTNTHGCRRIVVSRGRL Q9WU39 Mukherjee et al., 2014  
Influenza M2 M2 − 40LWILDRLFFKCIYRFFEHGLK Q20MD5 Sugrue et al., 1990; Holsinger et al., 1995; Veit et al., 1991  
RyR1 RYR1 Ryr1 14LRTDDEVVLQCSATVLKEQLKLCLAAEGFGNRL P11716 Chaube et al., 2014  
   110RHAHSRMYLSCLTTSRSMTDK P11716 Chaube et al., 2014  
   243RLVYYEGGAVCTHARSLWRLE P11716 Chaube et al., 2014  
   295EDQGLVVVDACKAHTKATSFC P11716 Chaube et al., 2014  
   527ASLIRGNRANCALFSTNLDWV P11716 Chaube et al., 2014  
   1030ATKRSNRDSLCQAVRTLLGYG P11716 Chaube et al., 2014  
   1664SHTLRLYRAVCALGNNRVAHA P11716 Chaube et al., 2014  
   2011HFKDEADEEDCPLPEDIRQDL P11716 Chaube et al., 2014  
   2227KMVTSCCRFLCYFCRISRQNQ P11716 Chaube et al., 2014  
   2316KGYPDIGWNPCGGERYLDFLR P11716 Chaube et al., 2014  
   2353VVRLLIRKPECFGPALRGEGG P11716 Chaube et al., 2014  
   2545EMALALNRYLCLAVLPLITKCAPLFAGTEHR P11716 Chaube et al., 2014  
   3160DVQVSCYRTLCSIYSLGTTKNTYVEKLRPALGECLARLAAAMPV P11716 Chaube et al., 2014  
   3392LLVRDEFSVLCRDLYALYPLL P11716 Chaube et al., 2014  
   3625SKQRRRAVVACFRMTPLYNLP P11716 Chaube et al., 2014  
Channel Subunit Gene Candidate S-acylation sites UniProt ID References 
Ligand-gated      
AMPA GluA1 Gria1 593FSLGAFMQQGCDISPRSLSGRI P23818 Hayashi et al., 2005  
   819LAMLVALIEFCYKSRSESKRMK P23818 Hayashi et al., 2005  
 GluA2 Gria2 600FSLGAFMRQGCDISPRSLSGRI P23819 Hayashi et al., 2005  
   826LAMLVALIEFCYKSRAEAKRMK P23819 Hayashi et al., 2005  
 GluA3 Gria3 605FSLGAFMQQGCDISPRSLSGRI Q9Z2W9 Hayashi et al., 2005  
   831LAMMVALIEFCYKSRAESKRMK Q9Z2W9 Hayashi et al., 2005  
 GluA4 Gria4 601FSLGAFMQQGCDISPRSLSGRI Q9Z2W8 Hayashi et al., 2005  
   827LAMLVALIEFCYKSRAEAKRMK Q9Z2W8 Hayashi et al., 2005  
GABAA γ2 Gabrg2 405QERDEEYGYECLDGKDCASFFCCFEDCRTGAWRHGRI P22723 Rathenberg et al., 2004; Fang et al., 2006  
Kainate GluK2 Grik2 848KNAQLEKRSFCSAMVEELRMSLKCQRRLKHKPQAPV P39087 Pickering et al., 1995  
nAChR α4 Chrna4 263TVLVFYLPSECGEKVTLCISV O70174 Alexander et al., 2010; Amici et al., 2012  
 α7 Chrna7 ND  Alexander et al., 2010; Drisdel et al., 2004  
 β2 Chrnb2 ND  Alexander et al., 2010  
NMDA GluN2A Grin2a 838EHLFYWKLRFCFTGVCSDRPGLLFSISRGIYSCIHGVHIEEKK P35436 Hayashi et al., 2009  
   1204SDRYRQNSTHCRSCLSNLPTYSGHFTMRSPFKCDACLRMGNLYDID P35436 Hayashi et al., 2009  
 GluN2B Grin2b 839EHLFYWQFRHCFMGVCSGKPGMVFSISRGIYSCIHGVAIEERQ Q01097 Hayashi et al., 2009  
   1205DWEDRSGGNFCRSCPSKLHNYSSTVAGQNSGRQACIRCEACKKAGNLYDIS Q01097 Hayashi et al., 2009  
P2X7 P2X7 P2rx7 361AFCRSGVYPYCKCCEPCTVNEYYYRKK Q9Z1M0 Gonnord et al., 2009  
   469APKSGDSPSWCQCGNCLPSRLPEQRR Q9Z1M0 Gonnord et al., 2009  
   488PEQRRALEELCCRRKPGRCITT Q9Z1M0 Gonnord et al., 2009  
   562DMADFAILPSCCRWRIRKEFPK Q9Z1M0 Gonnord et al., 2009  
Voltage gated      
Potassium      
BK, maxiK KCa1.1 Kcnma1 43WRTLKYLWTVCCHCGGKTKEAQKI Q08460 Jeffries et al., 2010  
   635MSIYKRMRRACCFDCGRSERDCSCM Q08460 Tian et al., 2008; 2010  
 Kv1.1 Kcna1 233SFELVVRFFACPSKTDFFKNI P16388 Gubitosi-Klug et al., 2005  
 Kv1.5 Kcna5 16LRGGGEAGASCVQSPRGECGC Q61762 Jindal et al., 2008  
   583VDLRRSLYALCLDTSRETDL-stop Q61762 Zhang et al., 2007; Jindal et al., 2008  
Sodium NaV1.2 Scn2a1 ND  Schmidt and Catterall, 1987  
   640MNGKMHSAVDCNGVVSLVGGP P04775 Bosmans et al., 2011  
   1042LEDLNNKKDSCISNHTTIEIG P04775 Bosmans et al., 2011  
   1172TEDCVRKFKCCQISIEEGKGK P04775 Bosmans et al., 2011  
Other channels      
Aquaporin AQP4 Aqp4 3DRAAARRWGKCGHSCSRESIMVAFK P55088 Crane and Verkman, 2009; Suzuki et al., 2008  
CFTR CFTR CFTR 514EYRYRSVIKACQLEEDISKFAEKD P13569 McClure et al., 2012  
   1385RRTLKQAFADCTVILCEHRIEA P13569 McClure et al., 2012  
Connexin Cx32 Gjb1 270GAGLAEKSDRCSAC-stop P28230 Locke et al., 2006  
ENaC ENaC β Scnn1b 33TNTHGPKRIICEGPKKKAMWFL Q9WU38 Mueller et al., 2010  
   547WITIIKLVASCKGLRRRRPQAPY Q9WU38 Mueller et al., 2010  
 ENaC γ Scnn1g 23PTIKDLMHWYCLNTNTHGCRRIVVSRGRL Q9WU39 Mukherjee et al., 2014  
Influenza M2 M2 − 40LWILDRLFFKCIYRFFEHGLK Q20MD5 Sugrue et al., 1990; Holsinger et al., 1995; Veit et al., 1991  
RyR1 RYR1 Ryr1 14LRTDDEVVLQCSATVLKEQLKLCLAAEGFGNRL P11716 Chaube et al., 2014  
   110RHAHSRMYLSCLTTSRSMTDK P11716 Chaube et al., 2014  
   243RLVYYEGGAVCTHARSLWRLE P11716 Chaube et al., 2014  
   295EDQGLVVVDACKAHTKATSFC P11716 Chaube et al., 2014  
   527ASLIRGNRANCALFSTNLDWV P11716 Chaube et al., 2014  
   1030ATKRSNRDSLCQAVRTLLGYG P11716 Chaube et al., 2014  
   1664SHTLRLYRAVCALGNNRVAHA P11716 Chaube et al., 2014  
   2011HFKDEADEEDCPLPEDIRQDL P11716 Chaube et al., 2014  
   2227KMVTSCCRFLCYFCRISRQNQ P11716 Chaube et al., 2014  
   2316KGYPDIGWNPCGGERYLDFLR P11716 Chaube et al., 2014  
   2353VVRLLIRKPECFGPALRGEGG P11716 Chaube et al., 2014  
   2545EMALALNRYLCLAVLPLITKCAPLFAGTEHR P11716 Chaube et al., 2014  
   3160DVQVSCYRTLCSIYSLGTTKNTYVEKLRPALGECLARLAAAMPV P11716 Chaube et al., 2014  
   3392LLVRDEFSVLCRDLYALYPLL P11716 Chaube et al., 2014  
   3625SKQRRRAVVACFRMTPLYNLP P11716 Chaube et al., 2014  

Common channel abbreviation and subunit as well as gene names are given. Candidate S-acylation sites: experimentally determined cysteine residues (bold) with flanking 10 amino acids. Underlines indicate predicted transmembrane domains. Amino acid numbering corresponds to the UniProt ID. References: selected original supporting citations.

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