Table 1. Effects on selectivity of amino... | Rockefeller University Press

Table 1.

Effects on selectivity of amino acids at positions 109, 112, and 116 in the S1 transmembrane segment of hH_V1

Construct	109	112	116	Selectivity	Reference
WT hH_V1	Val	Asp	Val	H⁺	Many
A113D	Val	Asp	Val	H⁺	This
D112E	Val	Glu	Val	H⁺	Musset et al., 2011
D112A/V116D	Val	Ala	Asp	H⁺	This
D112V/V116D	Val	Val	Asp	H⁺	This
D112V/V116E	Val	Val	Glu	H⁺	This
D112V	Val	Val	Val	0	Musset et al., 2011
D112V/V109D	Asp	Val	Val	0	This
D112A, N, S, H, K, F	Val	Ala	Val	Cl^–	Musset et al., 2011
D112A/V109D	Asp	Ala	Val	Cl^–	This
D112V/V116S	Val	Val	Ser	Cl^–	This
D112V/V116N	Val	Val	Asn	Cl^–	This

Construct	109	112	116	Selectivity	Reference
WT hH_V1	Val	Asp	Val	H⁺	Many
A113D	Val	Asp	Val	H⁺	This
D112E	Val	Glu	Val	H⁺	Musset et al., 2011
D112A/V116D	Val	Ala	Asp	H⁺	This
D112V/V116D	Val	Val	Asp	H⁺	This
D112V/V116E	Val	Val	Glu	H⁺	This
D112V	Val	Val	Val	0	Musset et al., 2011
D112V/V109D	Asp	Val	Val	0	This
D112A, N, S, H, K, F	Val	Ala	Val	Cl^–	Musset et al., 2011
D112A/V109D	Asp	Ala	Val	Cl^–	This
D112V/V116S	Val	Val	Ser	Cl^–	This
D112V/V116N	Val	Val	Asn	Cl^–	This

Selectivity to H⁺ means V_rev was close to E_H at various ΔpH; 0 means no credible currents; Cl⁻ means V_rev shifted negatively when Cl⁻ replaced CH₃SO₃⁻ in the external solution. Acidic residues are bold; neutral residues other than Val are italicized.

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